番茄细菌性茎枯病病原的鉴定

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中图分类号：S641.2；S436.412 文献标识码：A 文章编号：2095-5774（2025）06-0634-06

Pathogen identification of tomato bacterial stem blight

Luo Xiaohua

Agricultural andRural BureauofLianjiang County，Fuzhou City，Lianjiang，Fujian35o5oo，China

Abstract：【Objective】Toclarifythe pathogencausing bacterialstem blighton winter tomatoinLianjiang County，Fujian Province，providinga theoretical basis for thecontrolof this disease.【Method】Thepathogen was isolatedandpurified usingthe plate streaking method.Itspathogenicity was confirmed through Koch'spostulates.The pathogen's identity was determined by physiological and biochemical tests，as wellas16S rDNA genesequence analysis.【Result】Theresults showed thatseven bacterial strains were isolatedand purifiedfromdiseased tomato stems，and Koch's postulates confirmed that allseven strains were the pathogens causing this disease.The bacterialcellswere shortrod-shapedand Gram-stain negative.Based on 22 physiological and biochemical tests，such as poly- β -hydroxybutyrate（PHB）production，yellowgren fluorescence productionon King'sB（KB）medium，fructan production，arginine hydrolysis，oxidase test，and utilizationof gucose，xylose，cellobiose，maltose，lactose，trehalose，andglycinebetaineetal，aswellas16SrDNAgene sequenceanalysis，thebacterial strainswere identifiedasPseudomonas viridiflava.【Conclusion】The tomatobacterial stemblightdiseasesinFujianiscausedbyP.viridiflava.

Keywords：tomato; bacterial stem blight; pathogen identification; Pseudomonas viridiflava; gene identification

番茄蔬果兼用，是我国重要的经济作物。（剩余8814字）