基于ISSR分子标记的刺桐属植物种质遗传多样性分析及指纹图谱构建
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中图分类号：S685.99 文献标识码：A 文章编号：2095-5774（2025）06-0601-09
Analysis of genetic diversity and construction of fingerprinting in Erythrina germplasmusing ISSR molecularmarkers
Chen Qinghai
（Quanzhou City ForestPark Development Center，Quanzhou，Fujian 362Ooo， China）
Abstract：【Objective]This study aimed to evaluate the genetic diversity of Erythrina germplasm，constructDNA fingerprinting，andanalyzephylogeneticrelationshipsusing ISSR molecular markers，therebyproviding amolecular basis forgermplasmresource identification，conservation，and breeding utilizationwithin thisgenus.【Method】Using 26 Erythrinagermplasmsas materials，polymorphic primers werescreened fromISSR primers forPCRamplification.The POPGENE32and NTSYS-pcV2.1O softwares were employed to analyze genetic parameters（Ne，H，I）and perform UPGMAclustering，respectively，andDNA fingerprinting were constructed.【Result】Theresults showedthatatotalof12 ISSR primers with good polymorphism were screened，amplifying168clear bandsacrossthe26 Erythrina germplasms， with a polymorphism rate of 95.18% .Genetic diversity parameters revealed an average effective number of alleles （ Ne ）of 1.4423，Nei's gene diversity index（ ⋅H ）of0.2812，and Shannon'sinformationindex （ I） of0.4423，indicating relatively rich genetic diversity.DNA fingerprinting constructed using primersUBC834，UBC835，and UBC841 coulduniquely identify all26Erythrina germplasms.UPGMAclusteranalysiscategorized the tested materialsintofive majorgroupsata geneticsimilaritycoefficientof O.39.Thisclusteringshowedsomecorrelationwith morphological characteristicsand geographical originbutalsoreflectedpotential geneticexchangeposiblydue tohuman-mediated introduction. 【Conclusion】ISSR molecular markersare efective inrevealing thegenetic diversityofErythrina germplasmandare suitable forresource identificationandphylogeneticanalysis withinthe genus.TheestablishedDNAfingerprinting provide technical support for germplasm identification，variety rights protection，and resource management. Keywords：Erythrina; ISSR; genetic diversity; DNA fingerprinting; cluster analysis
刺桐属植物广泛分布于全球热带与亚热带地区，在我国主要分布于西南部至南部，有10多种栽培种[1-2]。（剩余8423字）
