没食子酸阻遏黄连介导的金黄色葡萄球菌抗生素交叉耐药研究

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中图分类号：R94 文献标志码：A

AbstractObjectiveTo investigate the inhibitory efect of gallic acid （GA）on the antibiotic cross-resistance in Staphylococcus aureus （SA）mediatedbyRhizoma Coptidis （RC）inthis study.MethodsSub-lethal dosesof Paeoniae Radix Rubra extract （PRRE）， GA，and the RGA solution， which mixed RC extract （RCE） with GA （1 mL RCE containing 3.6mgGA ），wereused for drug-exposure ofSA for 12 days，respectively.Then susceptibilityanalysis was performed by measuring the minimum inhibitory concentration （MIC）of the induced strains.16S rDNA sequencing was used to identify the induced strains andanalyze their homology.RT-qPCR was used toanalyze the expressionlevelofresistant genes in induced strains to explore the mechanisms of antibiotic cross-resistance.ResultsRPRE，GA，RGAinduced strains could not mediate the antibiotic cross-resistance in SA，respectively. Compared with RCE induced mutantstrins，theexpresinofgnes （smrB，nA，clp，isG，norA，fmtA_lemrB_1，4590，AicaR，BTN44_13995，HMPREF0776_1664 and vraG）in the RGA-induced strains，were down-regulatedby the fold change from 50.00% to 94.16% .Two genes bmr3 and BTN44_15030 were not detected due to their low expression levels. ConclusionsBoth RPR and its active monomer GA could not trigger the cross-resistance to antibiotics in SA，and GAcould inhibit the antibiotic cross-resistance mediated by RCE in SA.

Key WordsGallic acid; Inhibition; Rhizoma Coptidis; Staphylococcus aureus; Cross-resistance

黄连（RhizomaCoptidis，RC）是一种具有较强抗菌活性的中药材，其抗菌活性成分主要包括小檗碱、黄连碱、阿魏酸和绿原酸等。（剩余8776字）