羊边界病毒纳米PCR检测方法的建立

打开文本图片集

中图分类号：S858.26 文献标识码：Adoi：10.3969/j.issn.1002-204x.2026.03.007

文章编号：1002-204X（2026）03-0041-06

EstablishmentofNano-PCRDetectionMethod forBorderDisease Virus

YuYouli'，Wang Jiandongl*，Yang Yuqiu²，Zhou Pan² （1.InstituteofAnimal Science，Ningxia Academyof AgricultureandForestrySciences，Yinchuan，Ningxia750002; 2.School of Animal Scienceand Technology，Ningxia University，Yinchuan，Ningxia，750002）

Abstract[Objective]Theaim is todeveloparapid detectionmethod forborderdisease virus （BDV）.[Method] Specific primers were designed based on theconserved 5'UTR sequence of BDV.By optimizing the reaction system parameters （including annealing temperature，primer concentration，and nanoparticleadditionratio），a BDVNano-PCRdetection methodwas succesfully constructed.Thesensitivity，specificity，and clinical applicabilityof this method were comparedandanalyzedanda parallel verification was conducted with those of theconventionalPCR method.[Result] The optimized Nano-PCRcould stably amplifyaBDV-specific target of 210 bp，with a minimum detection limit of 1.1 × （20 10 copies/ μL ，and its sensitivity was 1OO times higher than that of the conventional PCR（1.1 ×103 copies/ μL ）. In the specific detection，this method showed no cross-reaction with bovine viral diarrhea virus，pestedes petits ruminants virus，and Caprine enterovirus.The detection of 5O clinical samples showed that thepositive detection rateof NanoPCR was 6% （3/50），which was completely consistent with the results of the conventional PCR，but the sensitivity was significantly higher than thatof theconventionalPCR.[Conclusion]theestablished BDVNano-PCR detection method has higher sensitivity and specificity than theconventional PCR，and can provide technical support for the early diagnosis and epidemiological monitoring of border disease virus infections.

Key Words Border disease virus; Nano-PCR; 5'UTR gene; Clinical diagnosis

羊边界病毒（borderdiseasevirus，BDV）是近年来国内新发的高致病性病原体，可引发山羊、绵羊等反刍动物呼吸道综合征、消化道溃疡及免疫抑制性疾病，导致约 30%～50% 幼畜死亡，严重威胁畜牧经济安全-5]。（剩余7582字）