黄孢原毛平革菌和长枝木霉对猪粪-玉米秸秆堆肥效果及微生物群落结构的影响

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中图分类号：S141.4 文献标志码：A 文章编号：1672-2043（2025）10-2639-14 doi：10.11654/jaes.2024-1007

Abstract：Inodertoachievetheeficientresourcetreatmentofagriculturalorganicwaste，theefectsofinoculatingPhanerochete chrysosporiumandTrichodermalongibrachiatumonnutrientchanges，humificationprocessandmicrobialcommunitysuccesiodring composting werestudied.Intisexperiment，pgmanureandcostrawereusedasompostingrawmaterials，adfourtreatmntswere setup：noinolatio（CK），oulatioofanerocetechspor（C）ricodealonibracatu）ndteirdulue （CM）.Bymeasuringinicatorssuchastemperature，pH，seedgminationidex（G），totaltrogen（T，totalposphorus，otal potassium（TK）andtotalutrientcontentduringthecompostingprocess，theffectsofdiferentexogenousmicrobialagentsonthe composting efectwerecompared.The high-throughputsequencing technologyof16SrRNAgenewasusedtoanalyzethediferential changesntheacterialommunitystrucuredringtheompostigprossihtheadditioofdiferenmicobialagnts.Itwasfoundtat theadditionofdifreticobialagentsouldpromotetetmperatuesefteompostpile.Tebcterialomuitystrucredidot changesignificantlyatdiferetsagsofachcompostingtreament，buttereweresignificantdiferencesinelatieabundancette samestage.Atthephylumlevel，therelativeabundanceofActinobacteriainthetreatmentwiththeaditionofthecompositemicrobial agentwas increased.Atthegenuslevel，thetreatmentwiththecompositemicrobialagentincreasedtherelativeabundanceof SMIAO2 in thelatertageofomposting.Pearsocorelatioanalysisshowedthatamongtefirst33genera，2generaweresigificantlyeae with temperature，pH，EC and E4/E6（P<0.05） . The results of RDA indicated that at the genus level，the changes in the bacterial community structureduringthecompostingprossithdiferent reatmentswerelatedtodiferentevironmentalfactorparameters，andeain environmental factors affecting the bacterial community structure were temperature and pH（ P<0.05 ）.In conclusion，the addition of microbialgentseiallpsiteobalntaeesityfcobaluityde maturity of the compost to a certain extent and increase the total nutrient content of the compost pile.

Keywords：agricultural waste;aerobic composting; microbial agent; humification; bacterial community

近年来，农业废弃物成为农业面源污染的关键源头之一。（剩余25150字）