基于代谢组学研究乙草胺对上海青生理代谢影响

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中图分类号：X503.23；X592 文献标志码：A 文章编号：1672-2043（2025）10-2499-09 doi：10.11654/jaes.2024-0929

Abstract：TosudytheimpactofcetochlontheyilogicaletabolismofteCinesecabbage，intissdyeexamindpact of acetochlor withvarious dosesonthepysiologicalchangesandmetabolitediferencesofChinesecabbagethroughphysiologicaland biochemical indices，as well as metabolomics analysis.The findings revealed that both low（ 5mg⋅L-1） and high（50mg⋅L-1） doses of acetochlorinducedoidativestresinCinesecabbage，evidencedbyignificantinreasinmalondialdehdelevelsndthactiiisf superoxidedismutaseandperoxidase.Thehighdosenotablyinhibitedphotosynthesis（chlorophyllcontentdecrease）andtotalprotein synthesis，ultimatelyleadingtogrowthinhibitioninChinesecabbage.Metabolomicsanalysisidentified148significantlyaltered metabolites.Linoleicacidandlinolenicacid，whicharecucialformaintainingcellmembranefluidityandregulatingosmoticprssure weresignificantlyumulatedioththehighandlowdosegroups.Inthelodoegroup，tereasanotableaccumulationofdefese relatedaminoids，fvodsdolccidsinaveschpoedtanteeespoetrssoeelye compoundsweregnificantlydcreasediteigoseroup，otentiallipaigteplantdptabilitytopesticdes.Fuoete highdoseofacetochlorsignificantlyipactedtheplantcarbonmetabolicpathwayleadingtoamarkedreductioninthelevelsofmost organicacidsandlysohosphatidylcholine，likelyssociatedwithecreasedpotosythesis.Itwassotathigandlowoctatios ofacetochlorcauseddiferentphysiologicalresponsesinChinesecabbageleaves，andChinesecabbageiitiateddiferentmetabolic defence mechanisms to different concentrations of acetochlor.

Keywords：metabolomics; herbicide; acetochlor; stress response; metabolic pathway

乙草胺是世界上最常用的低毒性除草剂之一，主要通过阻碍蛋白质合成抑制杂草生长，对于禾本科杂草和部分阔叶杂草具有很好的防治效果[]。（剩余16278字）