天香丹对o×-LDL诱导的内皮细胞自噬作用的影响

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AbstractObjective：Toinvestigate theefectofTianxiangdanonautopaginumanumblicalveinendothelialcels（UVCs）foloing oxidized low-densitylipoprotein（ox-LDL）injury.Methods：HUVECs was divided into control group，ox-LDLgroup，ox-LDL + Tianxiangdan group，and ox-LDL + rapamycin group. activity of HUVECs was detected using the CCK-8 method，the content of nitric oxide（NO） wasmeasuredbycolorimetry，thelevelofautophagywasassessedusingtheMDCmethodautophagicmorphologicalchanges were observedbytransmisionelectronmicroscopeandtheexpresonofautophagrelatedproteinsasdetectedbyWesternBlotethod. Resuts：cellviabitintheox-LDTianxiangdangroupandtheox-LDLrapamycingroupwassignificantlyhighethanthatinthe ox-LDLgroup，and cell permeabityandautophagy levelssignificantly increased in bothgroups（ P<0.05 or P<0.01 ）.numberof autophagosoeaaiagetofinaingddpsifoatd structuralproteinUNC-51-ikeKinase1（ULK1）andtheautophagproteinmirotubule-asociatedproteinlightchain3（LC3）-/3- increased.Conclusion：Tianxiangdanmayup-regulate ULK1andLC3-I/LC3-Iexpresson levelsbyinhibitingmTORsignal， promote ox-LDL-induced autophagy and improve cell viability.

Keywordsumanumbilicalveinendothelialcels;oxidizedlo-densitylipoprotein;Tianiangdan;autophagy;experimntalstudy

动脉粥样硬化（AS）是一种慢性多因素疾病，与心血管疾病的发展密切相关[1]。（剩余8907字）