抗虫转基因玉米对草地贪夜蛾的室内抗性水平鉴定

打开文本图片集

中图分类号：S435.132 文献标识码：A 文章编号：1006-060X（2025）03-0061-0

引用格式：，等.抗虫转基因玉米对草地贪夜蛾的室内抗性水平鉴定[J].湖南农业科学，2025（3）：61-66

DOI：10.16498/j.cnki.hnnykx.2025.003.011

Evaluation ofResistance ofInsect-Resistant Transgenic Maize to Spodoptera frugiperda inLaboratory

XIONG Mei， SONG Jun，WEI Chao，LI Hui， YANG Qiang，DAI Xiao-hang，LIU Wen-juan （InstituteofQualittandardndTestingTechnologyResearch，ichuanAcademyofAgricuturalSciences，Chengdu06

Abstract：With the transgenic maize carrying C r y I A b and thatcarrying Vip3Aa20 asdonors， Cry1Ab and Vip3Aa20 were introduced intothemaizecutivarXianda90l'byrossbreeding.Theesistanceofinsect-resistantransgenicmaizetoSodopterafrgiperdwas evaluatedbyinvitrotisseboassayandthecontentofexogenousproteiswasdeteredbyee-linkedimmunosobntaay Theresultsofbioassayandproteidetectionconfirmedeachother.Theresultsshowedthattransgenic'Xianda90l'stablyexpreed exogenousproteinsCryAbandVip3Aa2OathighlevelsinalltssuesatallstagesandhadstronglethaleffectonS.frugiperda， demonstratingapromising commercial application prospect.

Keywords：insect-resistanttrasgenicmaize;Spodopterafrugiperda;evaluationofresistanceinlaboratory;CryAb;ViAa

随着生物育种技术的迭代升级，转基因作物已在全球范围内迅速推广并投入应用。（剩余9917字）