砂藓SMPD基因的克隆及对干旱和高温胁迫响应分析

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中图分类号：Q943 文献标识码：A 文章编号：1000-3142（2025）08-1530-13

Abstract： Sphingomyelin phosphodiesterase（SMPD）plays a crucial role in the sphingolipid metabolism process by hydrolyzing sphingomyelin into phosphatidylcholinesandceramides.Toinvestigatethe functionsrelatedto drought toleranceandhigh-temperaturetoleranceofan SMPDgene（RcSMPD）inthestronglydesiccation-tolerant plant Racomitrium canescens，quantitative real-time PCR （qPCR）was employed to detect the expresion of RcSMPD under dehydration stress and high-temperature treatments.The coding sequence of RcSMPD was cloned，and bioinformatics analysiswascarredouttopredicttheprotein encoded byRcSMPD.Furthermore，transgenic Arabidopsis thaliana lines overexpresing RcSMPD were constructed to evaluate thedrought tolerance and high-temperature tolerance of RcSMPD.The results were as folows：（1）RcSMPD was found to respond tothe dehydration stress and high-temperature treatments of Racomitrium canescens to different degres.（2）RcSMPD had a total of 326 aminoacid residues and was a hydrophilic protein.itcould be an acidic or neutral SMPDand waspredicted tobelocated in chloroplastsor vacuoles.（3）Compared with wild-type plants，the morphology of the RcSMPD overexpression transgenic Arabidopsis thaliana showeddiferences，yetthere was no significant diference in stressresistance under simulated drought stress and high-temperature stress treatments among the A .thaliana lines.In conclusion，RcSMPD can participate inthe response of Racomitrium canescens to dehydration and high-temperature stress. Overexpresionof RcSMPD can change the morphologyofArabidopsisthaliana，buthasnosignificant impactonitsdroughttoleranceandhigh-temperature resistance.

Keywords：Racomitriumcanescens，sphingomyelinphosphodiesterasegne，expresionanalysis，droughtstres，ig temperaturestress

鞘磷脂（sphingomyelin，SM）于人类脑部中被发现并命名（Vesperetal.，1999），主要存在含丰富脂类组织的细胞膜和脂蛋白中（Harder&Simons，1997），其作为质膜的重要组成部分，以特定的排列方式构成膜中脂质区域的一部分（Adametal.，2002）。（剩余22259字）