二穗短柄草BdFKF1基因调控烟草开花的转录组学分析
打开文本图片集
中图分类号：Q943 文献标识码：A 文章编号：1000-3142（2025）08-1518-12
Abstract：FKF1is known tobeablue light-responsive geneandanimportantfactor in regulating plantflowering through the photoperiod pathway.To explore the molecular mechanismof BdFKF1 gene inregulating flowering in tobacco plants through he photoperiod pathway，wild-type tobacco （SR1）and BdFKF1 gene-transformed tobacco plants （BdFKF1-OE） were usedas materials.Transcriptomic sequencing andRT-qPCRvalidation were conducted to observe and record the flowering timein both materials.The results were as follows：（1）In SR1 vs FKF1 group，a totalof 472 diferentially expressed genes were identified，with 248 up-regulated genesand 224 down-regulated genes.Among them，14 diferentiallyexpressd genes wererelated tothephotoperiod，with7up-regulated genesand7down-regulated genes.（2）GO enrichment analysis revealed that diferentially expressed genes were significantly enriched in pathways such as U5 snRNP，oxidoreductase activity，acting on paired donors and response to blue light.Among them，the diffrentially expressed genesrelated to the photoperiod were mainly enriched in pathwayssuch as FK5O6 binding， photoperiodismand flowering，photoperiodism，phosphatidylethanolamine binding，macrolide binding，andregulationof flower development.（3）KEGG enrichmentanalysis revealed that the diferentially expressed genes were mainly enriched in pathways such asABC transporters，Protein processing in endoplasmic reticulum，cutin，suberin and wax biosynthesis and circadian rhythm-plant pathways.Among them，the diferentially expressed genes related to the photoperiod were mainly enriched in pathwayssuch as plant hormone signal transduction andcircadian rhythmplant.（4）Theobservation and recording showed thattheflowering timeof BdFKF1-OE plants was3.9dearlier than that of SR1.（5）RT-qPCRresults wereconsistent with the trend of changes inthe transcriptomic data，indicating that the transcriptomicdatahad highreliability.In conclusion，under long-dayconditions，the BdFKF1 genecanaffect the expresion of photoperiodpathway-related genes，andoverexpressionof theBdFKF1 gene promotes flowering in tobacco plants.
Key Words： FKF1 gene，photoperiodism，tobacco，Brachypodium distachyon， transcriptomics
植物开花过程被称为从营养生长到生殖生长的过渡期，该过渡期由植物精确调控，而调控植物开花的常见途径主要有光周期、春化、自主、环境温度、年龄和赤霉素6种，其中光周期途径是调节各种植物开花的重要环境因素之一（Songetal.，2015；Wang et al.，2020；Izawa，2021）。（剩余17653字）
