哈茨木霉T9131鉴定及其拮抗病原菌和诱导黄芪抗病的作用分析
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中图分类号：Q945.8 文献标识码：A 文章编号：1000-3142（2025）08-1508-10
Abstract：Thefungus Trichodermaspp.is ubiquitouslydetectableinsoil andplantrots，and it playsacrucial roleinthe biologicalcontrolof agriculture.To explore the influenceof Trichoderma spp.ontheresistanceof Astragalus membranaceus to rotrot，thisstudyisolatedandidentifiedTrichodermaspp.fromAstragalus membranaceusrootrot，andsubsequently investigated itsantagonisticactiononpathogenandanalyzed itsefectsonphysiological indexes of diseaseresistanceof Astragalus membranaceus，determined the typeof Trichodermaspp.throughmorphological characteristics，ITSand tef1 sequenceanalysis，analyzedantagonistic effects of Trichoderma spp.isolate on Fusarium solani HYFS-1 byplate confrontationassy.Thisstudyalso determined thephysiological indexessuch ascatalase（CAT），superoxidedismutase （SOD），phenylalanine ammonia-lyase（PAL），peroxidase（POD）and proline（Pro）to clarify the roleof Trichoderma spp.isolate ininducingthediseaseresistanceof Astragalusmembranaceus.Theresults wereasfollows：（1）TheTrichoderma spptype isolated from Astragalus membranaceus root rotwas identifiedas Trichoderma harzianumandnamed T9131.（2） Theinhibition rate of Trichoderma harzianum T9l31 against Fusarium solani HYFS-1 reached 72%±1% after6days of confrontation.（3）Compared to HYFS-1alone，T9131significantlyenhanced SODactivityat0hafter HYFS-1infection；at the 24 h HYFS-1 infection，T9131markedlyincreasedSODactivity，PODactivity，andProcontent;bythe48hHYFS-1 infection，T9131significantlyelevatedPODactivity，PALactivity，andProcontent.Tosumup，Trichodermaharziaum T9131 cancontrolAstragalus membranaceus rotrotbydirectlyinhibitingthe growthof HYFS-1andinducingtheactivities of SOD，POD，PALandProcontentof Astragalus membranaceus under Fusarium solani HFS-1 stres.Thisresearch will laythefoundationforexploring thebiocontroleffectofTrichodermaharzianumanditsmachanismofresistanceofAstragalus membranaceus root rot.
Keywords：Astragalus membranaceusroot rot，Trichoderma harzianum，Fusarium solani，antagonistic action， physiological index determination
黄芪（Astragalusmembranaceus）是豆科多年生草本植物，分为膜荚黄芪（A.membranaceus）或蒙古黄芪（A.membranaceusvar.mongholicus）两大类，以根入药，是药用价值很高的中药材（任小霞等，2016）。（剩余14443字）
