不同来源广藿香叶绿体基因组研究
打开文本图片集
中图分类号：Q943.2 文献标识码：A 文章编号：1000-3142（2025）04-0716-14
Chloroplast genome analysis Pogostemon cablin from different origins
XING Bingnan，LIANG Yingying，WU Wenru*，LU Yaru， ZOU Heyuan，PENG Xiaoqi （SchoolPharmaceutical Sciences，Guangzhou 51Ooo6，China
Abstract：Pogostemoncablinpossssssignificant medical and industrialvalues，as itcanbe usedfor medicinal purposes as well as for essential oil extraction. However，the yield and quality P . cablin can vary depending on the ecological environments andartificial cultivation measures employed in diferent production regionsandorigins.Inorder
to study the structural characteristics and compare the diferences the chloroplast genome P .cablindifferent origins，this study used the DNBSeq sequencing platform to sequence the whole genome P . cablin，used GetOrganelle to assemble the complete chloroplast genome，annotated the chloroplast genome on the CPGAVAS2 website，and analyzed the basic structural characteristics，IR/SC boundary comparison，genome comparison and collinearityanalysis， simplerepeat sequences and interspersed repeat sequences，diversityanalysis and relative usage analysis synonymous codons.The results were as follows：（1）The fullength the chloroplast genomes 2O different origins P .cablin was 152 461-152 510 bp，and 132 genes were annotated，including 87 CDSs，37tRNA genes and 8 rRNA genes.（2） The mVISTA comparison found that atpF，atpF-atpH，rps16-trnQ-UUG，rpoB-trmC-GCA，accD，psaI-ycf4，petApsbJ，rpl16，andrps15-ycfl were hypervariableregions.（3）Thesites withnucleicaciddiversitygreaterthanO.002 were located in the trnM-CAU-atpB interval，ycf4，rpl32，and rpl32-trmL-UAC interval.（4）Atotal 64codons encoding 20aminoacidsweredetected，andthere were 33 highly preferred codons，among which codons ending inA/Uaccounted forthemajority.（5）And74-76 SSRs，15-18 palindrome repeat sequences，and12-17forward repeat sequences were detected.（6）Genetic distance analysisand phylogeneticanalysis found that only GSY_MLXY hada distant genetic relationshipwithothercultivatedtypes.Inthisstudy，the genomestructure informationanddiferentsiteschloroplasts from 20 different sources P . cablin are obtained，which provides basic data for the development molecular markers and the screening superior germplasm.
Key words： Pogostemon cablin，chloroplast genome，repetitive sequence，diversity，relative synonymous codon usage
广藿香（Pogostemoncablin）为唇形科刺蕊草属植物，以干燥地上部分入药，其功效为芳香化浊、开胃止呕、发表解暑（国家药典委员会，2020），广泛应用在医药、食品和化妆品加工业等方面，具有较高的经济价值，亦是新型冠状病毒肺炎防疫中的重要中药（李浩等，2021）。（剩余15919字）