滇牡丹花瓣色斑形成的分子机制研究
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中图分类号：Q943 文献标识码：A 文章编号：1000-3142（2025）04-0628-13
Molecular mechanism of the colour spot formation in Paeonia delavayi petals
LI Haiqing'，DU Chun²，WANG Juan²*， ZHANG Huaibi³（1.ColegeofditUniversityKungO，a；3.ZedIsiutefornt&odsechLiedalestoto，Zd）
Abstract：To investigatethe molecular mechanisms of colour spotformation inPaeonia delavayi petals，this study used yellow petals of P .delavayi with and without colour spot as experimental material.Utilizing the Ilumina platform for transcriptome sequencing and the UPLC system for metabolome analysis，the keydiferentially expressed genes （DEGs）
and transcription factors affecting colour spot formation in P .delavayi were screened. The resultswere as follows：（1） The transcriptome sequencing yielded 63 981 Unigenes with an average length of 8 0 5 b p ，and 6 8 . 2 4 % of these Unigenes wereannotated.Atotalof19496DEGs wereidentified，of which41DEGs were involved inflavonoidbiosynthesisand DFR，CHS，and C H I structural genes showed significant differential expression. Among the obtained 37 MYB transcription factors，oneR2R3-MYB transcription factor，PdMYB3O，was found to playa significant role in promoting colour spot formation.（2）Targeted analysis using the UPLC-MS/MS platform detected 44 anthocyanin compounds.（3） The expresion trends of DEGs identified by RNA-seq were consistent with theqRT-PCR results.Insummary，the colour spot formation of P .delavayi is mainly influenced by anthocyanins.The transcription factor PdMYB3O ispositively correlated with the structural genes CHS， C H I ，and DFR during the yellow flower with red spot bud stage（B-S1） due to their similar highexpressonlevels.Itispredicted that PdMYB3Omayactasa positiveregulator in theflavonoid biosynthesispathway，enhancing theexpression levelsof structural genesinvolved inflavonoidbiosynthesis，thereby promoting theacumulationofanthocyanins intheplant.Thisstudyprovidesascientificbasis fordeveloping efficient breeding techniques for P .delavayi.
Key words：Paeonia delavayi，colour spot formation，transcriptome，metabolome，diferentially expressed genes （DEGs）
芍药属植物作为世界范围内享有盛誉的观赏植物，其来源于中国，拥有源远流长的栽培历史和育种传统（Zhouetal.，2014）。（剩余21930字）