增补无性系对湿地松种子园遗传多样性的影响

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DOI：10.11931/guihaia.gxzw202401008

中图分类号：Q943 文献标识码：A 文章编号：1000-3142（2025）04-0689-13

Effects of supplementary clones on genetic diversity of Pinus elliottii seed orchard

QIN Xianyu ， FENG Yuanheng²， XIE Junkang²，MENG Lanyang³，YANG Zhangqi （2 （1.CollegeofLifeSciees，GuangiNalUniersityGuilin5，Guangxi，China；2.GuangxiIstituteofForstcice TimberForestResearchIstitute，Nanning5302，China；3.NanningForestryResearchIstitute，Naing300，Ca）

Abstract：Supplementingclonesinseedorchardswith limited or mising clonesrepresents a crucial scientific management strategy.This research focusedonthe clonal seedorchardof Pinuseliotiiat Nanning Forestry Research Institute，examining howadditional clones influence theorchard's genetic diversitytoofer insights foritsscientific management.Utilizing 16 SSR markerpairs，thestudyassessed genetic diversityvariationspre-and post-clone augmentation，analyzing material geneticuniquenessand relationships through fingerprintand genetic clustering.The results were as follows：（1）Enhancing clone numbers from 18 to 5O led to a 2 . 3 7 % rise in polymorphic information content，a 1 4 . 0 7 % rise in average number of observed alleles，a 1 . 2 3 % risein average number of effective alleles，and a （204号 3 . 5 1 % rise inthe Shannon index.（2）It was determined that11 SSR marker pairs could identify all5O clones，for which fingerprnt maps of 5O clones were established.（3）The genetic distance among these clones varied between 0.018 and 0.670，and using a O.251 threshold，the UPGMA clustering diagram grouped them into seven categories. In conclusion， it is considered that clone supplementing can enhance the genetic diversity of P .elliottii seed orchards，but the extent of improvement is limited.Developing fingerprints ofers a dependable method for identifying premium P elliottii clonesand analyzing seed orchard progeny parentage.The genetic clustering of established clones serves asa referene for parent selection in P .eliottii hybridization experiments，efficiently preventing inbreeding.

Key Words：Pinus elliotii，EST-SSR，genetic diversity，clonal expansion，genetic breeding

湿地松（Pinuselliottii）原产于北美东南沿海、古巴、中美洲等地。（剩余20277字）