棉隆熏蒸对十字花科根肿病土壤微生物群落结构影响

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关键词：棉隆；熏蒸；高通量测序技术；土壤微生物群落结构；多样性

中图分类号：S435.6 文献标志码：A 文章编号:2097-2172(2025)06-0572-07

doi:10.3969/j.issn.2097-2172.2025.06.014

Effects of Dazomet Fumigation on Soil Microbial Community Structure in Relation to Clubroot Disease of Cruciferous Crops

JING Zhuoqiong,XU Shengjun, Sun Qian, Guo Zhijie (Institute of Plant Protection, Gansu Academy of Agricultural Sciences,Lanzhou Gansu 73oo7o, China)

Abstract:Theefectsof dazometfumigationonthemicrobialcommunitystructureofcruciferous clubrotsoil were studied aimingtoprovideatheoreticalbasisfortheaplicationofdazometfumigationincontrolingsoil-boediseasesofcruciferous vegetables.High-throughputsequencing technologywasemployedtoanalyzethesoil microbialcommunitystructureafterdazomet fumigationinclubroot-infectedsoil.Resultsshowedthattheabundanceoffungiandthediversityofbacteriainthesoilwere significantlyreducedbysoilfumigation,andtheOUTs(operationaltaxonomicunit)clusteringresultsofbacteriaandfugiby β diversityanalysisshowedthatthfumigationsignificantlychangedthestructureofmicrobialcommunitiesinthesoil.Thecommunity structuresimilarityusingtwodiferentconcentrationsoffumigationtreatmentwashigh,butshowedlowsimilaritytountreatedand water-treatedcontrolsamplescolltedonthedayofmembraneremoal.Inadition,teuseofdiferentconcentrationsoffuigation (375,400kg/ha)couldalsoincreasetheabundanceofbeneficialfungisuchasChaetomiumandTetracladium,andreducethe abundanceofpathogenicfungisuchasAlternariaandFusarium.However,fumigationalsohadanegativeimpactoncertain beneficial soil fungi.The highest increase inabundance of Chaetomium was 6.69% ,and the highest decrease in Alternaria was 8.84% ：

Key Words:Dazomet; Fumigation; High-throughputsequencing technology; Soil microbial community structure; Diversit

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