冰冻切片技术在水稻茎秆显微结构上的应用

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中图分类号：Q944.6 文献标志码：A DOI：10.3969/j.issn.1007-7146.2025.04.003

Cryo-sectioning Technique for Microstructure Studies on Rice Stalks

YANG Shuibo， GONG Zhengya， ZHANG Xiaoyan，LI Xiaoyun， LYU Lefu （School ofAgricultureandBiotechnology，Sun Yat-sen University，Shenzhen518io7，China）

Abstract： Inorder toobservethemicroscopic structure andlignin distributionofrice stalks efficientlyandconveniently， this studyadoptedthe natural drying-cryo-sectioning methodandsuccessullyobtained thintissuesections.Bycombining the autofluorescencepropertiesofplantsandusing confocalmicroscopy technique，the microscopic structureofrice stalks was observed.This technicalmethodmainlyimprovedthecryosectioning techniqueforricestalks.Aftericestalkswerenaturally dried foracertainperiodof time，they weredirectlyembeddedandsectioned.Sectionscanbeprepared withor withoutsafranin staining toobtainmicroscopicsections withrelativelyintactstructures.ubsequentlyultravioletlight，bluelight，dgen lightwereusedtoexcite theautofluorescenceofricestalks，followedbymicroscopicimagingandphotography.Theresearch results showed that the improved cryosectioning technique could produce tissue sections with a thickness of 1μm and nearly intactcellstructures.Byusingalaserscanningconfocal microscope tocollectredfluorescence signals excitedby greenlight， themicroscopicstructureandlignin distributionofricestalk sectionscanbeobservedquicklyandclearly.Thisstudyprovides references forthe research on the tisse structures and the explorationof the physiological functions inPoaceae plants.

KeyWords：ricestalk;cryosectiontechnique;confocal microscopytechnique;cellularmicrostructure;ignindistribution （ActaLaserBiologySinica，2025，34（4）：304-311）

冰冻切片技术是一项在低温条件下使组织冻结到一定硬度后进行切片的技术，多应用于组织细胞的快速观察、组织化学研究和分子生物学研究等领域[1]。（剩余13671字）