不同秋眠级紫花首蓿响应低温胁迫的转录组学分析

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Abstract：Thecultivationand production of Medicago satiua L.are affected byabrupt cold and extreme low temperature，which seriously afect the healthy development offorage industry in China.Inthis study，two cultivars of alfalfa with diferent fall dormancy，‘Xinjiang Daye’ and‘Zhaodong'，were treated with low temperature for （2号 and ， and cold response genes were screened by transcriptome sequencing technology. After of low temperature treatment，the cold resistanceof‘Zhaodong’was significantly higher than that of‘Xinjiang Daye’at 192 h. Through transcriptome sequencing analysis at and ， 13 differentially expressed genes related to cold resistance were further screened. After qRT-PCR fluorescence quantification was verified，7 genes with expression patterns consistent with the transcriptome data were identified： 2 genes with opposite expression trends in the two varieties of alfalfa，and 5 genes with the same expresion trend in the two varieties ofalfalfa. Inaddition，KEGG pathway enrichment analysis was performed on all diferentially expressed genes，and11 metabolic pathways related to cold resistance including starch and sucrose metabolism，MAPK signaling pathway and galactose metabolism were identified.The diferential genes and metabolic pathways screened in this study would provide references for subsequent studies on cold resistance mechanism and molecular breeding of alfalfa.

Key words：Alfalfa；Low temperature；Cold hardiness； Gene response； Metabolism response

紫花苜蓿（MedicagosatiuaL.）是一种优质的多年生豆科牧草，具有蛋白质含量高、消化率高、生长快、环境适应性强等优良特性，且能够再生并多年利用，在我国畜牧产业中发挥着不可或缺的作用。（剩余21093字）