高效液相色谱法测定镶制品中苯并(a)芘含量的研究

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中图分类号：TS201.1 文献标志码：A 文章编号：1000-9973（2025）05-0196-05

DOI：10.3969/j.issn.1000-9973.2025.05.028

Study on Determination of Benzo（a） pyrene Content in Naan Products by High-Performance Liquid Chromatography

QIAO Gui-fang，FENG Zuo-shan，AN Bi-fang，CHEN Chuang-ye， TAO Yong-xia* （College of Food Science and Pharmacy， Xinjiang Agricultural University，Urumqi 830o52，China） Abstract：Benzo（a）pyrene （Bap）is a carcinogenic substance that is easily found in food and is harmful to human health. In order to rapidly determine the content of Bap in Naan products，in this study，the method for the rapid determination of Bap by high-performance liquid chromatography（HPLC） is optimized.The sample is extracted with acetonitrile. Under the conditions of PLATISILTM ODS liquid chromatography column （250mm×4.6mm ， 5μm ）；mobile phase：acetonitrile：water is 88：12 ；flow rateis 1mL/min ，column temperature is 24.5∘C ，injection volume is 10μL ，fluorescence detector： excitation wavelength is 384nm and emission wavelength is 406nm ，the content of Bap is determined，and the standard curve is drawn. The regression equation is Y=1 047 259X+101 946 ，with R2=0.9995 . The average spiked recovery rates are between 68.58% and 95.10% ， the RSD values range from 0.69% to 1.38% ， the limit of detection of benzo（a） pyrene is 0.09μg/kg ，and the limit of quantification is 0.31μg/kg The Bap content in four kinds of Naan products is determined. The results show that the content of Bap all does not exceed the national limit of 2μg/kg . This method is easy to operate，efficient，with high recovery rate and reliable results，and it is suitable for the determination of Bap in Naan products.

Key words： high-performance liquid chromatography；Naan products； benzo（a） pyrene； fluorescence detector；benzo（a）pyrene molecularly imprinted column

镶制品是指用小麦粉、食用植物油、生活饮用水等为主要原料制成的具有新疆地方特色的面制品之一，也是新疆人民最喜爱的食物之一[1-2]。（剩余10419字）