鸡腿菇蛋白提取工艺优化及其抗氧化活性的研究

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DOI：10.3969/j.issn.1000-9973.2025.05.005

中图分类号：TS201.1 文献标志码：A 文章编号：1000-9973（2025）05-0029-08

Optimization of Extraction Process of Protein from Coprinus comatus and Study on Its Antioxidant Activity

ZHU Min1，2，3 ， FENG Yi-kai1，2，3， LI Meng-kai1，2，3， YANG Yan-jun1，2.3* （1.College of Food Science and Engineering， Shanxi Agricultural University， Jinzhong O308O1，China; 2. Department of Biological Science and Technology， Jinzhong University， Jinzhong O3o619，China; 3. Shanxi Composite Condiment Technology Innovation Center， Jinzhong O3o6l9，China）

Abstract： In order to determine the optimal extraction process of protein from Coprinus comatus by alkali extraction and acid precipitation method and analyze the antioxidant activity of Coprinus comatus protein，in this paper，with defatted Coprinus comatus as the raw material and protein extraction rate as the index，the effects of liquid-solid ratio，alkali extraction time，alkali extraction temperature and pH on the extraction rate of Coprinus comatus protein are studied. With ferrous ion chelation capacity， DPPH free radical scavenging capacity and hydroxyl free radical scavenging capacity as the indexes，the antioxidant activity of Coprinus comatus protein is evaluated. The results show that the optimal extraction process is liquid-solid ratio of 22：1 ，alkali extraction time of 126min ， alkali extraction temperature of 48°C and pH of 12. At this time，the extraction rate of Coprinus comatus protein is 18.49% Within the mass concentration range of 2～10mg/mL ，the ferrous ion chelation capacity，DPPH free radical scavenging capacity and hydroxyl free radical scavenging capacity of Coprinus comatus protein increase with the increase of mass concentration，and the antioxidant capacity also increases，indicating that Coprinus comatus protein has certain antioxidant activity. This study has provided a certain reference basis for the further development and utilization of Coprinus comatus resources.

Key words： Coprinus comatus protein；alkali extraction and acid precipitation method；response surface method; extraction rate;antioxidant activity

毛头鬼伞（Coprinuscomatus）亦称鸡腿菇[]，是一种味美、营养丰富的食用菌。（剩余12808字）