环指蛋白1和阴阳1结合蛋白可能通过影响上皮间质转化途经抑制膀胱癌细胞增殖、迁移

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ABSTRACT：ObjectiveTo investigate the effects of RING 1and YY1binding protein（RYBP），a member of the polycomb group（PcG），on bladder cancer cel proliferation and invasion ， as well as the underlying mechanisms involved，so as to provide referencefor the preventionand treatment of bladder cancer.MethodsOverexpresing and knocking down of RYBP were achievedin bladdercancercellines（T24，UM-UC-3，and 5637）via plasmidsor siRNAs.Cell proliferation was assessedvia thiazolylblue（MTT）andcolony formation assys，whereas migrationwas evaluated via sratch and Transwellassys.Changes in epithelial-mesenchymal transition（EMT）markers and otherrelated proteins wereexamined with Western bloting.Results RYBP overexpression significantly inhibited bladercancercell proliferation，invasion，and migration，whereasRYBP knockdown promoted these behaviors.Western bloting resultsrevealed thatRYBP overexpresion downregulatedthe expressionsofEMTmarkers N-cadherin，Vimentin，and Slug，utupregulated the expresionofE-cadherin.Conversely，YBP knockdown upregulated theexpressions of N-cadherin，Vimentin，and Slug，whilereducing theexpressionof E-cadherin. ConclusionRYBPappears toinhibit the proliferation and migrationof bladdercancer cels viatheEMTpathway，indicating its potential application in bladder cancer therapies.

KEY WORDS：bladder cancer;RING 1and YY1 binding protein（RYBP）;polycomb group;epithelial-mesenchymal ransitior

摘要：目的探讨多梳族群蛋白家族（PcG）的成员环指蛋白1和阴阳1结合蛋白（RYBP）对膀胱癌细胞增殖和侵袭性的影响及其相关机制，为膀胱癌的防治提供基础研究参考。（剩余11274字）