蓝光通过脑-肾轴对大鼠肾结石形成的影响研究

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ABSTRACT：ObjectiveTo analyze the effects of blue light on the formation of kidney stones.MethodsA total of 40 rats were randomly divided into 4 groups：A，B，C，and D，with 10 rats in each group.Rats in groups C and D were administered with a mixture of 10 g/L ethylene glycol，20 g/L ammonium chloride，and 100 g/L calcium gluconate via gavage （2 mL per mouse），while rats in groups A and B received an equal volume of physiological saline via gavage.From the second day after gavage，rats in groups A and C were subjected to twice-daily blue light irradiation （one hour per session） as an intervention，while rats in groups B and D were subjected to fluorescent lamp irradiation using the same method.After 4 weeks of intervention，the 24-hoururine samples were collected，and the rats were then euthanized for the collection of blood and kidney tissue samples.Serum levels of antidiuretic hormone （ADH），urinary Ca2+，and urinary oxalate （Oxa） were measured.Levels of malondialdehyde （MDA） and superoxide dismutase （SOD） in kidney tissues were detected using ELISA.Von Kossa staining was performed to observe pathological changes in kidney tissues and the presence of calcium salt crystals in the kidneys.Results Compared with groups A and B，groups C and D showed higher accumulation of calcium salt crystals in renal tissues，as well as elevated levels of ADH，urinary Ca2+，urinary Oxa，and MDA in renal tissues， additionally，the SOD level in renal tissues was lower （P<0.05）.Compared with group D，group C exhibited higher accumulation of calcium salt crystals in renal tissues，along with increased levels of ADH，urinary Ca2+，urinary Oxa，and MDA in renal tissues；conversely，the SOD level in renal tissues was lower （P<0.05）.ConclusionBlue light may increase the formation of kidney stones in rats by promoting the secretion of ADH in serum and oxidative stress in kidney tissues through the brain-kidney axis.

摘要：目的分析蓝光通过脑-肾轴对大鼠肾结石形成的影响。（剩余7633字）