ROS响应型水凝胶的制备及其抗菌性能研究

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中图分类号：R978.1 文献标志码：A 文章编号：1001-8751（2025）03-0194-10

Study on the Preparation and Antibacterial Properties of ROS-Responsive Hydrogels

.ong Zi-linl，Wang Zi-qi'，MaJunl，Huang Xiao-jing²，LeiLei²，GuHuan’， Zeng Ruil.3，Gou Kai-jun34 （1 The College of Pharmacy and Food Science of The Southwest Minzu University， Chengdu 610041; 2Chengdu Institutefor DrugControl&NMPAKeyLaboratoryforQualityMonitoringandEvaluationof Traditional Chinese Medicine （Chinese Materia Medica）， Chengdu 610000; 3 Key Laboratory of Research and Application of Ethnic Medicine Processng and Preparation on the Qinghai Tibet Plateau， Southwest Minzu University，Chengdu 610225; 4 College of Grassland Resources， Southwest Minzu University， Chengdu 610225）

Abstract： ObjectiveTo screen the optimal ratio of the PVA/BSP hydrogel matrix to prepare rifampicin loaded ROS-responsive hydrogel，improve the bioavailabilityofrifampicin，evaluate itsROS-responsive drug release and antibacterial efect，and provide a new optionfor bacterial infected wounds.MethodsTheappropriateconcentration ratio of PVA/BSP hydrogel matrix was screenedand optimized，and the ROS-responsive hydrogel was prepared by loadingrifampicin.TheROS-responsive drug release characteristicsof the hydrogel were measured by ultraviolet spectrophotometer，and its antibacterial property was further evaluated in vitro.ResultsThe optimal PVA/BSP ratio was 2：3 （ 8%PVA ， 12%BSP ）.The adhesion strength of the rifampicin-loaded hydrogel was 56.83KPa at a 15 mm stretch distance，and the highest compressive strength was 96.42 781 KPa.Compared with the PBS-released group， the rifampicin hydrogel showed a higher cumulative drug release in the H2O2 -PBS solution，and the rifampicin release rate at 48h （92.86%） ）Was significantly higher than that in the PBS-released group （47.53%） .Invitro antibacterialtestresultsshowed thatthe inhibition Zoneof the hydrogel to methicilin-resistant Staphylococcus aureus， Staphylococcus aureus， and Escherichia coli was 3.4±0.10cm ， 1.75±0.05 cm and 1.8±0.01cm ，respectively. ConclusionRifampicinhydrogel has goodbiodegradability，tisueadhesion，mechanical properties，ROS-responsive drug release ability，and goodantibacterial properties.Therefore，rifampicin hydrogel has a broad applicationprospect in the antibacterial and repair of bacterial infection wounds.

Key words： ROS response；rifampicin；hydrogel; in vitro release；antibacterial

皮肤将人体的内部环境与外部环境分开，作为人体的天然屏障在防御外界的伤害中起着至关重要的作用[1-2]。（剩余16093字）