淫羊藿素骨靶向脂质体的制备及表征研究

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中图分类号：R944.2 文献标志码：A 文章编号：1001-8751（2025）03-0187-07

Preparation and Characterization of Bone Targeting Icaritin Liposomes

Lai Jin-mei¹， Zhang Shuang-qing²，LiuLi-han³ （1 ShenzhenLuohu Maternity and Child HealthcareHospital， Shenzhen 518000; 2 National Institute forNutritionand Health，Chinese Center for DiseaseControland Prevention，Beijing100050; 3 School of Pharmaceutical Sciences， Southern Medical University， Guangzhou 510515）

Abstract： ObjectiveTo prepare icaritin （ICT）-loading bone targeting liposomes and characterize in vitro properties.MethodsThe bone targeting peptide CSDsSD was synthesized by solid-phase peptide synthesis，and covalently linked to DSPE-PEG2000-MAL to obtain DSPE-PEG2000-CSDSSD.ICT liposomes were synthesized by membrane dispersionusing egg yolk lecithin，cholesterol，DSPE-PEG2oo-CSDsSD，and the ICT.The particle size and potential of ICT liposomes were measured using a Malvem instrument. The drug loading and encapsulation efficiency of ICT liposomes were analyzed using UV-Vis absorbance spectroscopy.ResultsThe ICT liposome had a hydrodynamic particle size of 225.3±1.8nm and a Zeta potential of -20.8±0.5mV. The transmission electron microscope image showed that ICT liposomes were uniformly sized spherical particles with a dry particle size of below 30nm . ICT liposomes did not significantly alter in hydrodynamic particle size or polydispersity over the course of a week， maintaining a particle size of about 225nm and a polydispersity index of less than O.26. The drug loading of ICT in ICT liposomes was 9.87% ，and the encapsulation efficiency was 68.90% .ConclusionWe have successfully prepared bone targeting liposomes with good stability， drug loading and encapsulation efficiency.

Key words： icaritin; liposomes； bone targeting; preparation; characterization

淫羊藿素（Icaritin，ICT）是小檗科淫羊藿属植物淫羊藿的活性成分淫羊藿苷的酶降解产物和淫羊藿黄酮类化合物的肠道代谢物[1]。（剩余8144字）