光倒刺鲍背部肌肉组织转录组测序及其特征分析

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中图分类号 S917.4 文献标识码A 文章编号 1007-7731（2025）12-0063-06

DOI号 10.16377/j.cnki.issn1007-7731.2025.12.016

Transcriptome sequencing and characteristic analysis of dorsal muscle tissue inSpinibarbushollandi

LI SixunLAI Jie DENG Binhua YE Shuzheng LIN Shengyue CHEN Weijian GUILin LI Qiang （School of Life Sciences， Guangzhou University， Guangzhou 51Ooo6， China）

AbstractToexplorethetranscriptomiccharacteristicsof Spinibarbus holandi，three femaleandthree maleindividuals （ 200g each） were used as experimental materials for highthroughput sequencing and subsequent analysis of the transcriptome. The results showedthata totalof95231190clean reads were obtained，which were assmbled into91467 Unigenes with atotallengthof73474804 nt.Among these，49O61Unigenes wereannotated inprotein databases，while48912 Unigenes wereannotated intheNRdatabase.The highest gene matching rates for Spinibarbus hollandi werewith Sinocyclocheilus rhinocerous （26%）， Sinocyclocheilus anshuiensis （23 % ）， and Sinocyclocheilus grahami （18 % ）. A total of 17557 Unigenes were anotated inthe GOdatabase，covering 49 functional subcategoriesacross threemajor biological categories：biological processes，cellularcomponents，andmolecularfunctions.Aditionall，28503Unigens were classfedinto25KOGclustes， including signal transduction mechanisms and general function prediction.Among the 25927 Unigenes annotated in the KEGG database，enrichment analysis revealed 295 metabolic pathways，with higher proportions observed in pathways such asligand-receptor interactions inneuroactive stimulationandthe MAPK signalingpathway.Microsatelitecharacteristic analysisdetected936microsatelitelociwithdinucleotiderepeatsbeing thmostabundant （5204），followedbytriucleotide （2523）and tetranucleotiderepeats （965）.Thetranscriptomicdataobtained inthis studyprovideavaluablereferencefor research on germplasm resources，genetic structure，molecular markerdevelopment，and genetic diversity exploration in Spinibarbushollandi.

Keywords Spinibarbus hollandi; transcriptome; gene annotation; characteristic analysis

光倒刺鲍（Spinibarbushollandi）属鲤形目（Cypriniformes）鲤科（Cyprinidae）鲍亚科（Barbinae）倒刺鲍属（Spinibarbus），广泛分布于长江以南各水系，是重要的经济鱼种之一，其抗病性较强，肉质鲜美，营养价值较高[1-2]。（剩余8313字）