新型MWCNTs滴鼻法有效构建了肺炎及纤维化小鼠模型

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中图分类号：R392-3 文献标志码：A 文章编号：1672-1098（2025）01-0099-10

Abstract：Objective The aim is to develop a new reproducible MWCNTs exposure mouse model，which is convenient，fast，economical reusable.Inorder to provide more eficient model support forthe futureresearch mechanistic exploration pulmonary fibrosis drugs.Methods 80μL （ 125μg/mL ）MWCNTs suspension was exposed in C57BL/6 mice for 14 days assessed their physiological status.Lung lesions in mice on days 3，7，14， 28 wereanalyzed byELISA，HE staining，Masson staining immunluorescence double staining.Finally，the experimental data were analyzed clustered byusing a new exposure scoring system for lung injury CNTs.Results The pulmonary inflammation formed on day 7， the fibrosis formed on day 14 after exposure. Only on day 28 did pulmonary fibrosis reach itsworst severity the mice were in their worst physiological state.Compared with theoropharyngeal perfusion method，the nasal infusion model greatlyreduced the formation time extensive fibrosis.MWCNTs Exposure increased the specific process FMT related markers（ α -SMA Col-I） the proinflammatory factor TGF -β1 through the activation the TGF-β1/Smad2/3 signaling pathway.Clusteranalysis defined the pathophysiological process thismodel as：initial stage，inflammatory phase，progressive stage fibrotic stage.Conclusion This study addressed the critical importance repeated nasal drops in lung induction inflammation generation fibrosis in CNT-exposed patients by establishing a novel mouse model MWCNTs exposure.

Key Words： Nasal drops ; pulmonary fibrosis ；carbon nanotubes ;model building

碳纳米管（Carbonnanotubes，CNTs）是一种应用广泛的纳米材料，可大规模生产。（剩余12582字）