基于 N,N. -二甲基丙烯酰胺聚合物溶液流变性能及其在毛细管电泳分离DNA中的应用
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中图分类号：063 文献标志码：A
Rheological Properties of Polymer Solution Based on N， N. -Dimethylacryla mide and Its Application in DNA Separation by Capillary Electrophoresis
MIAO Guilan1， CHEN Xuefeng²， ZHANG Jibao²，LUBin1，NUSRULLAHMehtab1， GONG Yunhan1， ZHU Liangyu1，FAN Qianyun1，WANG Yanmei1 （1.School ofChemistry and Materials Science， Universityof Science and TechnologyofChina，Hefei 230026， China; 2. Nanjing Suyuan Gene Technology Co Ltd， Nanjing 211899， China）
Abstract： The homopolymer （PDMA） of N. N. -dimethylacrylamide （DMA） and the copolymer （P（DMA-co-AM）） of DMA andacrylamide （AM）were synthesized byaqueous solution polymerization.Theefects of intrinsicviscosity，the molarratio of AM to DMA，temperature and shear rate onthe rheological behaviorsof polymer solution were studied，and the corresponding polymer solution was used as a separation mediumforDNAfragments analysis using capilaryelectrophoresis.Thestudy of rheological behaviors show that with increasing theAMcontent the intrinsic viscosityof P（DMA-co-AM） increased，andtheviscosityoftheDNAseparationsolutionformedbythepolymerinthebufersolutionincreased.Whenthe temperature increased from 25°C to 60∘C at aconstant shear rate， the viscosity of PDMA separation solution with higher molecular weight decreased by 57.0% ，while the viscosity of P（DMA-co-AM） solution with higher molecular weight decreased byabout 50.8% ：Compared with the PDMA solution，P（DMA-co-AM） solution could better resist the environmental temperaturechange.Theresultsof DNA fragment analysisshow thatwith increasing theAMcontent，the P（DMA-co-AM） solution with higher molecular weight obtained a higher resolution on the separation of big fragmentsof DNA and beter reproducibility compared with the P（DMA-co-AM） solution with lower molecular weight.
Key words： N☉ NΩ -dimethylacrylamide;polymer solution; rheology； capilary electrophoresis； DNA fragmentsseparation
毛细管电泳（Capillary Electrophoresis，CE）作为一种高效分离技术，在生物医学与化学分析领域具有重要的应用价值[1，其基于带电粒子在电场作用下迁移率的不同实现组分分离，兼具分离度高、分析速率快、检测灵敏、微量进样及自动化等优势[2.3]。（剩余13433字）
