针康结合促进C57/BL6J小鼠脑缺血后星形胶质细胞转分化为神经元

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Electroacupuncture combined with rehabilitation training improves neurological function of mice with cerebral ischemia by promoting astrocyte transdifferentiation

TANGDongning1，KANGYunyun，HEWenjie3，XIA Qing，2

ResearchCenterxprimtaluptureoloediclcolojinesityieicij

China;³DongguanRehabilitationExperimentalSchool，ongguan53ooo，Cina

Abstract：Objective To exploretheeffectsofacupuncture combined withrehabilitation training forpromoting transdiferentiationofastrocytes intoneuronsinmiceaftercerebralischemia.Methods MaleC57BL6Jmice weresubjectedto intracerebralmicroinjectionof anadeno-assciated viruscarrying the GFAPpromoterfor NeuroD1and Ngn2 overexpression intheastrocyes，folowed3or1dayslaterbylectrocoagulationofthedistalmiddleerebralateryAftermodelingtemice wererandomlydividedintomodel group withoutinterventionsandinterventiongrouptreatedwithelectroacupunctureatthe acupointsBaihui （GV20），left Hegu （LI4），Neiguan （PC6）， Zusanli （ST36），and Yanglingquan （GB34） 24h after surgery. The mice inte intervention group were housed individually incages withrunning wheels，andtheiractivitywasrecorded every 24h Neurological function scores of the mice were assessed on the 1st， 14th， and 21st days after modeling. Transdifferentiation of astrocytes inthetarget brain regions was observed using double immunofluorescence staining.Results Compared with thoseinthemodel group，the micereceiving eletroacupunctureandrehabilitationtrainingshowedsignificantimprovementof neurologicaldeficitsat14and21daysafter modeling.TheGFAPpromoteroftheAAV2/5vectorspecificalllabeledthelocal astrocytes，andcomparedwiththatthatinthemodel group，thenumberofAAV-positivecelscolabeledwiththeneuronal marker DCXsignificantlyincreasedafter14daysof electroacupunctureandrehabilitationintervention，andthenumberof AAV-positivecelscolabeledwiththeneuronalmarkerNeuNsignificantlyincreasedafter21daysofintervention.Concusion Inmicewithcerebralischemia，electroacupunctureandeabilitationtrainingcanpromotetransdierentiationofatrocytes intoneuronsintheischemicbrainregion，andtheeficiencyoftransdifferentiationispositivelycorelatedwiththe improvement of motor function.

Keywords： astrocytes;cerebral ischemia;transdiffrentiation; electroacupuncture combined withrehabilitationtraining

星形胶质细胞是中枢神经系统占比最多的细胞之一，占中枢神经系统细胞总数的 20%～50% ，与神经元来源于同一类祖细胞，且遗传谱系接近，能够维持发育、正常生理和衰老过程中的中枢神经系统稳态2。（剩余17323字）