水稻对恶苗病菌侵染响应的蛋白质组分析

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中图分类号：S511；Q816 文献标志码：A 文章编号：1008-0864（2025）06-0126-10

ProteomeAnalysisofRiceResponsetoGibberellafujikuroi Infection

SHAO Lihua，LI Peng （Instituteof PlantProtection，HeilongjiangAcademyofLandReclamationSciences，Harbin15oo38，China）

Abstract： Bakanae disease，a common and highly harmful fungal disease of rice，seriously impacts rice yield.To clarifytheup-regulateddiferential proteins of high-resistance varieties inresponse to the bakanaepathogen，the TMT（tandem mass tag）quantitative proteomics technology was employed to compare the proteomes before and after infection with the bakanae pathogen，and the significantly differentially expressed proteins with P⩽0.05 and a fold change （FC）>1.2 or <0.83 were screened.The resultsshowed that 124up-regulatedand75 down-regulated differentially expressed proteins were screened.The 124 proteins with up-regulated expression were subjected to gene ontology（GO）enrichment analysis，and got 88 enrichment items，among which 4 items were significantly enriched， including 2 biological processes （response to stress and response to oxidative stress），2 molecular functions （peroxidase activityand hemebinding），which should be beneficial toremove peroxides in rice bud tissueand had a protectiveefect oncels.Atotalof45 pathways were obtainedby（kyoto encyclopediaof genes and genomes KEGG） enrichment analysis，and3 pathways were significantly enriched for upregulated differential proteins including phenylpropanoidbiosynthesis，thebiosynthesis of secondarymetabolitesand phenylalaninemetabolism， respectively，whichshould be beneficialtoimprove diseaseresistanceof rice.Therewere3differential proteins （Os01t0327400-01，Os12t0112000-01，Os01t0963000-04）were common among 4 itemsof GO significant enrichmentand3pathways ofKEGG significant enrichment，which couldbethe keyproteins inthebakanaedisease resistance.Above results provided scientific theoretical basis for the research of disease resistance.

KeyWords：rice；bakanae disease；Gibberella fujikuroi species complex；proteome；disease resistance

水稻恶苗病又名徒长病，俗称公稻子，其病原菌有性态最初被命名为藤仓赤霉菌[Gibberellafujikuroi（Sawada）Wollenw]，无性态命名为串珠镰孢菌（Fusariummoniliforme），近年来将恶苗病菌有性态更名为藤仓赤霉复合种（Gibberellafujikuroispeciescomplex，GFSC），该病是危害性强的水稻常见真菌性病害，广泛分布于世界各个稻区，在水稻生育后期发病重的地块发病率达10%＼～20%，严重影响水稻产量[1-2]

化学农药能有效杀死种子表面的病原菌或抑制其活动，控制种子传病，降低苗期发病率，最大限度地控制病害的再侵染，降低大田产量损失。（剩余8957字）