干旱胁迫下小粒咖啡SNP位点与可变剪接分析
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图分类号：S571.2 文献标志码：A 文章编号：1008-0864（2025）06-0072-11
Analysisof SNPLoci and Alternative Splicing Events in Coffea arabicaL.UnderDrought Stress
YU Haohao 1，2 ，DONG Xiangshu1，ZHAO Hao1，LI Zhongxian²，HU Faguang²，LI Yanan 2 LOUYuqiang²，HE Feifeil* （1.SchoolofAgriculture，YunnanUniversity，Kunming65o5o，China；2.IstituteofTropicalandSubtropicalCashCros Yunnan Academy of Agriculture Science，Yunnan Baoshan 678ooo，China）
Abstract：To enrich the information on single nucleotide polymorphism（SNP）lociand elucidate theroleof alternative splicing（AS） events inresponse to drought stressin Coffea arabicaL.，C.arabica cultivar‘Catimor’was employed as the experimental material for drought stresstreatment for7and14d，respectively.RNA-seq technology combinedwith bioinformatics methodswasutilizedtostatisticallyanalyzethedrought-resistant SNPlociandAS events in C .arabica.Theresults showed thata total of 265O47 SNP loci were identified inallthe samples，with a transition rate of 60.72% and a transversion rate of 39.28% ，distributed on11 chromosomes，with the most SNPs on Chr.2.The numbers of SNPloci located inexons and intronsof thegene were 110582and100 936，respectively. Sequence anotation revealed thatthe SNPloci were mainly enriched inaminoacid synthesis，transport and carbon metabolism.TheresultsofASeventanalysis showedthat3809and4142diferentASevents were identified inthe comparison groups of2 drought stages （O dof drought vs7dof drought；7 dof droughtvs14dof drought）， respectively.Among them，SE was found to bethe most prevalent splicing mode.The genes responsible for these AS events showed significant assciations with spliceosome and mRNA surveillance pathway.Through comparative analysis between differential expression genes and AS genes，4 splicing factors （LOC113699257，LOC113712703， LOC113741326 and LOC113730764）consistently demonstrated significant up-regulation under drought stress conditions.These drought-induced splicing factorsmight playcrucialroles inregulatingcofeeresponse todrought stress orroot development.Above results laid a foundation for further revealing the molecular mechanism of C arabica in response to drought stress.
Key Words：Coffea arabica L.;drought stress；SNP loci; alternative splicing
小粒咖啡（CoffeaarabicaL.）是茜草科咖啡属常绿灌木或小乔木，具有喜温凉、喜潮湿、不耐旱的特点，是继石油之后排名世界第二的原料型产品，在全球热带亚热带地区广泛种植-2]。（剩余16090字）