红掌高效离体培养技术研究

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中图分类号：S682.14 文献标识码：A

文章编号：2095-5774（2025）04-0394-07

Abstract：【Objective】Theeficient in vitro propagation technology of Authurium andraeaum‘Pink Champion’ was studiedinordertoprovideacertainexperimental basis forthefactoryseedling.【Method】Theeffectsofthedifrent explantsonthecalusinductionandtheeffectsofthediferentculture mediaoncallusdifferentiation，proliferationand rooting were studied byusing the aseptic seedlings of Authurium andraeaum 'Pink Champion’ as the experimental materials.Result】 Cultured for 6O days in the same induction medium，thecallus inductionrate of stemsegments （ 93.33% ）was thehighest.When thecallus was cultured for 6O days and9O days，the differentiationrate of adventitious buds in the medium of MS + 0.25-0.50 mg/L 6-BA was significantly higher than that of MS +1.OO mg/L 6-BA.The callus cultured for9Odays withoutcutting werere-inoculatedontheold formula medium for1OOdays，thediferentiation coeficientofadventitious budsandthedrymaterproliferationcoeffcientinthe mediumofMS+O.25mg/L6-BAwere the highest. The rooting rate of 1/2 MSbasic medium was significantly higher than that of MS basic medium.And the rooting rate of rootless seedlings reached 100% when cultivated in the medium of 1/2 MS+0.20 mg/LNAA + 1.0O g/L activated carbon（AC）for12days.【Conclusion】Inthe eficient in vitro propagation of Authurium andraeaum‘Pink Champion'， stem segments were most suitable for inducing callus.The medium of MS+0.25mg/L 6-BA was most suitable for callus differentiation and proliferation.The medium of 1 12MS+0.20mg/L NAA +1.00g/L AC was most suitable for rooting.

Keywords：Anthurium andraeaum; stem segments; tissue culture; in vitro propagation

红掌（AnthuriumandraeaumLind.）又名安祖花，为天南星科花烛属多年生常绿草本植物，花型独特，叶姿优美，是著名的盆切两用型热带观赏植物。（剩余9324字）