1株多谱系重组猪繁殖与呼吸综合征病毒的分离鉴定及遗传进化分析

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中图分类号：S852.651 文献标志码：A 文章编号：1004-390X（2025）04-0030-10

Abstract： [Purpose] To investigate the genetic evolution of porcine reproductive and respiratory syndrome virus （PRRSV）. [Methods] Blood samples from diseased pigs and lung tissues from dead pigs were collected from a suspected porcine reproductive and respiratory syndrome outbreak pig farm in Anhui Province. RT-PCR， indirect immunofluorescence assay， virus growth curve and bioinformatics software were used for virus isolation， identification and gene sequence analysis.

[Results] A strain of PRRSV was isolated from Marc-145 cels and designated as AH1. The complete genome of AHl was 15022 bp in length， which shared 90.0% nucleotide identity with the NADC30 strain from USA. The virus titer was 105.67mL-1 . Phylogenetic analysis based on the whole genome showed that AH1 and NADC30-like strain were on the same branch of the genetic evolutionary tree，and had characteristic insertion or deletion of amino acids in NSP2 region similar to NADC30- like strain. The tenth amino acid of GP2 protein was mutated from L10 to S10. Recombinant analysis showed that PRRSV AHl was a recombinant strain with NADC30 as the backbone and PRRSV CHla， JXA1 and QYYZ strains providing recombinant fragments. [Conclusion] Strain AHl results from multiple recombinations among PRRsV lineage 1， lineage 3 and lineage 8， and new virulenceassociated amino acid mutations occur at the same time. This study provides a new reference for the study of the epidemic distribution and genetic variation of PRRSV recombinant strains in China.

Keywords： porcine reproductive and respiratory syndrome virus; NADC3O-like; recombinant virus; isolation and identification; genetic evolution

猪繁殖与呼吸综合征（porcine reproductive andrespiratory syndrome，PRRS）是由PRRS病毒（PR-RSvirus，PRRSV）引起的高度传染性疾病[1。（剩余10659字）