电针百会、大椎对5xFAD模型小鼠认知障碍及ROCK2/PI3K/AKT信号通路的影响

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中图分类号：R285.5 文献标志码：A 文章编号：1007-3213（2025）11-2798-09

DOI： 10.13359/j.cnki. gzxbtcm.2025.11.025

Abstract：Objective To investigate the effcts of electroacupuncture at Baihui （GV2O） point and Dazhui（GV14） point oncognitive function and potential mechanisms in Alzheimer’s disease（AD） mice.Methods Forty-eight male five-transgenic familial Alzheimer’s disease （5xFAD） mice were divided into four groups：empty vector group，knockdown group， empty vector + electroacupuncture group，and knockdown + electroacupuncture group. The knockdown group received Rho-associated coiled-coil containing protein kinase 2（ROCK2） knockdown via adeno-associated virus（AAV） microinjection technology.Knockdown eficiency was verified by polymerase chain reaction（PCR），and gene expression levels were confirmed by Western Blot.After 4 weeks of viral expression and transfection，electroacupuncture intervention at Baihui and Dazhui acupoints was administered for 4 weeks. Cognitive function wasassessed using behavioral tests.Pathological products，inflammatory markers [interleukin 6 （IL-6），tumor necrosis factor α （TNF- ∇⋅α∝ ）]， and changes in the PI3K/AKT signaling pathway were detected using immunofluorescence staining，enzyme-linked immunosorbent assay（ELISA），and Western Blot techniques. Results Compared with the empty vector group，the ROCK2 knockdown group showed significantly improved spatial and contextual memory （ P<0.05 or P<0.01 ），reduced amyloid deposition and inflammatory indicators （ P<0.05 or P<0.01 ），and increased expression levels of phosphorylated protein kinase B （P-AKT）and phosphorylated phosphatidylinositol-3-kinase（P-PI3K）（ P<0.01 ），while the knockdown + electroacupuncture group exhibited more pronounced effects than knockdown group P<0.05orP<0.01 ）.Conclusion Electroacupuncture at Baihui point and Dazhui point can regulate ROCK2 to activate the PI3K/AKT signaling pathway，improve cognitive impairment in AD mice，reduce Aβ deposition，and alleviate inflammatory responses.

Keywords：electroacupuncture；Alzheimer’s disease；Baihui（GV2O）；Dazhui（GV14）；Rho-associated coiled-coil containing protein kinase 2；PI3K/AKTsignaling pathway；Aβ deposition；inflammatory responses; 5xFAD mice

阿尔茨海默病（AD）是一种导致认知能力和记忆力下降的神经退行性疾病，其发病率呈逐年上升的趋势[1-3]。（剩余13317字）