金花葵果实不同组群抗糖尿病作用

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关键词：金花葵果实；2型糖尿病；PI3K/AKT信号通路；腺苷酸活化蛋白激酶；小鼠中图分类号：R285.5 文献标志码：A 文章编号：1007-3213（2025）07-1765-08DOI：10.13359/j.cnki.gzxbtcm.2025.07.030

Anti-diabetic Effects of Different Components of Hibiseu ManihotL.Fructus

PANYongchen，CHEN Liping，LIN Shaodan，XIONG Xiubi，LI Guangying，WU Jiewei，YUAN Jie（Guangzhou University of Chinese Medicine，Guangzhou 51ooo6 Guangdong，China）

Abstract：Objective To investigate the therapeutic efects and mechanisms of Hibiseu Manihot L.fructus on type 2 diabetes melltus（T2DM） mice.Methods T2DM mice were randomly divided into normal group，model group， positive drug group，Hibiseu Manihot L. fructus total extract group，water-solube part group，and Hibiseu Manihot L.fructus ethyl acetate fraction group，with 1O mice in each group.After4 weeks of treatment，antidiabetic effects were evaluated by monitoring body mass，fasting blood glucose（FBG），oral glucose tolerance test （OGTT），fasting insulin（FINS），homeostasis model assessment of insulin resistance（HOMA-IR），and area under the blood glucose curve （AUC）.Liver tissue status was assessed through liver index and hematoxylin-eosin （HE）staining.Results Compared with the model group，the water-solube part and total extract group showed significant improvements in mental status，food intake，body mass，excretion，and the FBG elevation was effectively suppressed，OGTT，FINS，AUC，HOMA-IR and liver index were significantly decreased （ P<0.05 （20 or P<0.01 ），the histopathological analysis revealed improved liver tissue morphology.Regarding channel protein expression，compared with the model group，the water-solube part group exhibited significantly upregulated phosphorylation levels of protein kinase B（AKT）and phosphatidylinositol 3-kinase（PI3K） in mouse liver tissue （ P<0.05 ），furthermore， the positive drug group， total extract group， and water-solube part group all demonstrated markedly increased phosphorylation of adenosine monophosphate-activated protein kinase （AMPK） in hepatic tissues （ P<0.05 ）. Conclusion The water-solube part of fructus of Hibiseu Manihot L fructus.efectively alleviates T2DM symptoms（polyphagia，polydipsia，polyuria，weight loss）and glucose metabolism disorders， with hepatoprotective effects potentially mediated through PI3K/AKT pathway activation and enhanced p-AMPK expression.

Keywords：Hibiseu Manihot L.fructus；type 2 diabetes melitus （T2DM）；PI3K/AKT signaling pathway; adenosinemonophosphate-activated proteinkinase;mice

2型糖尿病（type2diabetesmellitus，T2DM）是以胰岛素分泌不足和胰岛素抵抗为主要特点的慢性代谢疾病，具有较高的发病率和死亡率[1-2]。（剩余10507字）