定坤丹通过microRNA-30d-5p靶向调节Smad2促进多囊卵巢综合征卵巢颗粒细胞增殖的机制研究

打开文本图片集

关键词：定坤丹；多囊卵巢综合征；转化生长因子β1；Smad2；microRNA-30d-5p；卵巢颗粒细胞；大鼠中图分类号：R285.5 文献标志码：A 文章编号：1007-3213（2025）07-1750-07DOI：10.13359/j.cnki.gzxbtcm.2025.07.028

Mechanism of Dingkun Dan in Promoting Ovarian Granulosa Cell Proliferation in Polycystic Ovary Syndrome via microRNA-30d-5p-Mediated Targeting of Smad2

CHEN Lan，YAN Xiaojing，LIAN Yuanpei，ZHU Li，ZHOU Shikang，WANG Dijun，CAI Jiali，YIN Chunyan，CAO Ji（Meng Research Institute，Changzhou Hospitalof Traditional ChineseMedicine，Changzhou213O03 Jiangsu，China）

Abstract：Objective To investigate the therapeutic mechanism of Dingkun Dan （DKD） in polycystic ovary syndrome（PCOS） by examining the effcts of microRNA-3Od-5p on ovarian granulosa cells（GCs）.Methods A PCOS rat model was established using dehydroepiandrosterone（DHEA）.Normal rat GCs and PCOS rat GCs were cultured in vitro and divided into four groups：blank control group，model group，low-dose DKD group，and high-dose DKD group.After grouping，GCs viability was assssed using the methyl thiazolyl tetrazolium（MTT） assay，GCs apoptosis was analyzed by flow cytometry，and the gene expression of transforming growth factor β1 （TGF-β1）， Smad2， Smad3，and microRNA-30d-5p in GCs was measured by real-time polymerase chain reaction（RT-PCR），protein expresion of TGF-β1，Smad2，and Smad3 in GCs was detected by Western Blot. Results Compared with the blank control group，the model group exhibited significantly decreased GCs viability， increased GCs apoptosis，upregulated mRNA and protein expresson of TGF-β1，Smad2，and Smad3，and downregulated microRNA-3Od-5p expression，the differences were statistically significant （ P<0.01 ）. Compared with the model group，both low- and high-dose DKD groups showed increased GCs viability，reduced GCs apoptosis， downregulated mRNA and protein levels of TGF- ⋅β1 Smad2 and Smad3，elevated microRNA-30d-5p expression，and the differences were statistically significant （ P<0.05 or P<0.01 ）.Conclusion DKD promotes GCs proliferation by targeting Smad2 via microRNA-30d-5p，suggesting a potential therapeutic role in PCOSrelated ovulatory dysfunction.

Keywords： Dingkun Dan； polycystic ovary syndrome （PCOS）； transforming growth factor β1 ；Smad2;microRNA-3Od-5p；ovarian granulosa cells；rats

多囊卵巢综合征（polycysticovarysyndrome，PCOS）是育龄妇女最常见的内分泌疾病，在一般人群中患病率高达 20%[1] 。（剩余12462字）