肾病I号方对慢性肾衰竭大鼠肾纤维化及FAK-Ras-MAPK通路的影响

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关键词：肾病I号方；肾衰竭；肾纤维化；胶原蛋白V；FAK-Ras-MAPK信号通路；大鼠中图分类号：R285.5 文献标志码：A 文章编号：1007-3213（2025）07-1744-06DOI：10.13359/j.cnki.gzxbtcm.2025.07.027

Effects of Nephropathy Formula I on Renal Fibrosis and FAK-Ras-MAPK Pathway in Rats with Chronic Renal Failure

WU Qianjia'，CHENG Jinguo²，LIN Chongze'，YANG Yang' （1.Wenzhou HospitalofTraditionalChineseMedicine，Wenzhou 3250Zhejiang，China；2.TheFirstAfiliatedHospitalof Wenzhou Medical University，Wenzhou 325Ooo Zhejiang，China）

Abstract：Objective To investigate the therapeutic effects and mechanisms of Nephropathy Formula I（NF-I） in rats with chronic renalfailure（CRF）.Methods A CRF model was established using continuous adenine gavage. After successful modeling，the rats were randomlydivided into normal group，model group，low-dose and highdose of NF-I groups，with 10 rats in each group.The group-specific interventions were administered.Renal histopathological changes were observed via hematoxylin-eosin（HE）staining，renal fibrosis was evaluated using Masson staining，collgen IV（Col-IV） expression in renal tissues was detected by immunohistochemistry，and protein expression offocal adhesion kinase（FAK），Ras，and mitogen-activated protein kinase （MAPK） in renal tissues wasanalyzedvia Western Blot.Additionally，in vitro experiments were performed to validate theeffectof NF-I on the FAK-Ras-MAPK signaling pathway.Results Compared with the normal group，the model group exhibited a significant increase in renal interstitial fibrosis areaand elevated protein expresson levels of Col- IV ， FAK，Ras，and MAPK in renal tissues，and the diferences were statistically significant （ P<0.05 ）.In comparison to the model group，both the NF-I low-dose and high-dose groups showed significant reductions in renal interstitial fibrosis area and downregulation of Col- IV ，FAK，Ras，and MAPK protein expression levels， and the differences were statistically significant （ P<0.05 ），while the effcts in the high-dose group were superior. In vitro validation results demonstrated that，compared with the control group，the protein expression levlesof cellular FAK， Ras and MAPK were decreased in drug-containing serum group （ P<0.05 ）.Conclusion NF- I effectivelyamelioratesrenal fibrosis in CRF rats，potentially through suppressionof the FAK-Ras-MAPK signaling pathway.

Keywords：Nephropath Formula I；renal failure；renal fibrosis；collagen IV；FAK-Ras-MAPK signaling pathway；rats

本课题组前期临床研究表明：“肾病I号”融滋肾、温阳、益气、化、利湿为一体，可以调节免疫功能，减少炎症介质分泌，用于治疗慢性肾炎获得满意疗效。（剩余6964字）