新型纳米探针通过靶向MN受体抑制透明细胞肾细胞癌的应用研究

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Application of innovative nanoprobes for targeted inhibition of clear cell renal cell carcinomaviaMN receptors

ZHU Hanwen， YAN Yishuo， QI Xin，LI Cong，WANG Ziqi DepartmentofUrology，HarbinedicalUniversityCancerHospital，Harbin5oo81，China Corresponding author： WANG Ziqi， Email： wangziqi@hrbmu.edu.cn

【Abstract】 Objective To evaluate the role of novel nanomaterial MN receptor targeted inhibition system （MTIS） in targeting and inhibiting clear cell renal cell carcinoma （ccRCC）. Methods The microprobe MTIS was designed and implemented for use with ccRCC cell lines 786-O and Caki-1. The targeting efficacy of both MTIS and MN was examined through laser scanning confocal microscopy （CLSM）. CCK-8 and Transwell assays were conducted to confirm the inhibitory impact of MTIS on ccRCC.Additionally， the Renca cell line was utilized to induce subcutaneous tumors in mice， and MTIS was administered through tail vein injection to assess its therapeutic effect. Results The CLSM observations revealed that MTIS exhibited excellent colocalization with tumor cells. The results from CCK-8 and Transwell experiments further demonstrated that MTIS significantly inhibited the migration and invasion of tumor cells. Furthermore，the effective concentration of MTIS injected via tail vein at the tumor site in mice was observed using the in vivo imaging system （IVIS）.Notably， the tumor volume in mice after 14，21 and 28 days treated with MTIS were markedly smaller compared to the control group （P<0.05） .Conclusion The novel nanoprobe MTIS effectively targets cRCCcelsand demonstrates asignificant inhibitory effecton their migration and invasion.

【Keywords】Clear cellrenal cell carcinoma； Tumor imaging； Nanomaterials； Targeted inhibition； MN receptor

肾细胞癌（renalcell carcinoma，RCC）发病率在全球范围内呈上升趋势，主要与人口老龄化、高血压、肥胖和环境暴露等因素有关[。（剩余11380字）