川陈皮素调节FAK/AKT信号通路对喉鳞状细胞癌细胞增殖和凋亡的影响

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中图分类号：R739.65文献标志码：A DOI：10.11958/20250433

Abstract：ObjectiveToinvestigate theefect ofnobiletinon the proliferationandapoptosis oflaryngeal squamous cellcarcinoma （LSCC）cellsand its mechanismrelated tothe focaladhesion kinase （FAK）protein kinase B（AKTsignaling pathway.MethodsTU177celsof LSCC were randomly assigned into thecontrol group，the L-nobiletin group（10 mg/L） group，the M-nobiletin group（ 20mg/L ），theH-nobiletin group（ 40mg/L ）and the H-nobiletin+FAK activator ZINC40099027 group 40mg/L nobiletin+2.5 g/L ZINC40099027）. CCK-8 method was used to detect cell viability. Flow cytometry wasused todetectcellapoptosis.Scratchexperimentwasused todetectcellmigration.Transwell methodwasused to detectcellinvasion.Western blot assaywasused todetect apoptosisandFAK/AKT pathwayrelated proteins.Results Compared with thecontrol group，thecellviabilityscratch healingrate，numberof invasivecels，the protein expreionsof Bcl-2，p-FAK/FAK and p-AKT/AKT were decreased in a dose-dependent manner in the L-nobiletin group，the Mnobiletin groupandthe H-nobiletin group，whilethecellapoptosisrateand proteinexpressionsofBax andcaspase-3 were increased in a dose-dependent manner （ P<0.05 ）. The OD value， scratch healing rate， number of invasive cells，Bcl-2， pFAK/FAK and p-AKT/AKTprotein expresionlevelswere higherin the H-nobiletin+ZINC40099027 group thanthoseof the H-nobiletingroup，andcellapoptosisrate，Baxand caspase-3protein expression were lower compared tothe H-nobiletin group （ P<0.05 ）.ConclusionNobiletin may inhibit the proliferationand promote the apoptosisof LSCC cellsby suppressing the FAK/AKT signaling pathway.

KeyWords：laryngealneoplasms;carcinoma，squamouscell;nobiletin;focaladesionprotein-tyrosineinases;proto oncogene proteins c-akt; cell proliferation; apoptosis

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