云南冷凉山地72份苹果种质资源遗传多样性分 析及分子身份证的构建

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中图分类号：S661.1 文献标志码：A 文章编号：1002－2910（2025）06-0022-06

Genetic diversity analysis and molecular identification card construction of 72 apple accessions in the cool mountainous areas of Yunnan

ZHAO Nafei'，LIULin，MAJun，HUANG Wenjing，LIUDandan（1.Yunnan Uversityunmng，Yunan，ina;IstittefHorticulturalCros，YnAcdeo AgriculturalSciences，Kunming，Yunnan650504，China）

Abstract： China boasts abundant apple germplasm resources，encompassing a large number of wild species，semi-wild species，and cultivated varieties.However，the widespread phenomena of synonymy （different names for the same germplasm） and homonymy （the same name for different germplasms） in production have hindered the conservation and utilization of germplasm resources as well as the development of the apple industry.In this study，genetic diversity analysis was conducted on 72 apple varieties （lines） collected from Zhaotong，Yunnan Province.Molecular identification cards were constructed based on the genotypes derived from fingerprinting，and molecular QR codes enabling rapid and accurate identification were also developed.This research provides a theoretical basis for the identification of apple germplasm resources.

Key words： apple; germplasm resources; SSR-PCR; genetic diversity; molecular ID card中国西南地区苹果资源丰富，有很多野生苹果 类型及栽培品种，但由于地势和气候的原因，研究很少。（剩余7569字）