外源三十烷醇对干旱胁迫下红豆草生长和生理特性的影响

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中图分类号：S541.4 文献标识码：A 文章编号：1007-0435（2026）01-0142-12

Abstract：To investigate the efects of exogenous triacontanol（TA）on the growth and physiological characteristics of sainfoin（Onobrychis viciifolia） seedlings under drought stress，three materials（‘Gansu’sainfoin， new line P1，and 2O49）were subjected to -1.0MPa polyethylene glycol 6000（ T1. ）simulated drought stress using a nutrient solution sand culture method. Foliar sprays of TA at O（CK）， 2.5（T2） ， 5.0（T3） ， 7.5（T4） ，10.0 （20号 （T5） ，and 12.5（T6）μmol⋅L-1 were applied，and 21 morphological and physiological indicators were measured toidentify the optimal TAconcentration.Results demonstrated that allTA concentrations aleviated drought stress，with 10.0μmol⋅L-1 TA T5） exhibiting the greatest efficacy.Under T5 treatment，shoot height，biomass，and photosynthetic parameters increased by 19%-454% compared to T1 ，while root-shoot ratio，osmoregulatory substances，and antioxidant enzyme activities decreased by 15%-89% . Principal component analysis identified stomatal conductance，chlorophylla，and carotenoidsas key regulatory indicators.Comprehensive drought resistance evaluation ranked treatmentsas T5>T6>T4>T3>T2>T1 ，with varietal responsiveness ordered as GS>P1>2049 . These findings indicated that TA enhanced sainfoin drought resistance by modulating physio-biochemical traits，with 10.0μmol⋅L-1 （ T5） being the optimal treatment，providing a theoretical basis for TA application in drought management during sainfoin cultivation.

Key words： Triacontanol; Sainfoin;Drought Stress;Physiological indicators;Photosynthetic parameters

受全球升温影响，我国干旱地区面积持续扩大，导致农业经济遭受重大损失1。（剩余18534字）