补播模式对喀斯特地区草地土壤养分和酶化学计量特征的影响

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中图分类号：S812.2 文献标识码：A 文章编号：1007-0435（2026）01-0087-11

Abstract：To investigate the effects of reseeding patterns on soil nutrients and enzyme stoichiometry ofdegraded graslands，a study was conducted in the Dushan of Guizhou，China，using a degraded grassland（CK）as the control. Two reseeding treatments were established： oat（Avena sativa L. ） + ryegrass （Lolium perenne L.） （AL） and oat + ryegrass + clover（Trifolium L.） （ALT）. The results showed that both AL and ALT significantly increased the contents of organic carbon，total nitrogen，and available phosphorus in the 0～10cm soil layer，with increasing by 42.66% and 57.29% ， 75.54% and 116.30% ，and 90% and 155.59% ，respectively. Under the ALT treatment， the C/N ratio in the 0-10cm soil layer significantly decreased，while the ΔN/Ω Pratio increased，alleviating soil nitrogen deficiency. The activities of β -1，4-glucosidase and β -1，4-N-acetylglucosaminidase significantly increased by 182.21% and 186.85% ，respectively，with a higher increase than that in the AL treatment. ALT significantly increased the enzyme vector length in the 0～10cm soil layer， intensifying microbialcarbon limitation.Correlationanalysis indicated that extracelular enzymeactivities were mainly regulated by thecombined efects of total nitrogen and organic carbon.In conclusion，theALTreseeding patterncould effctively improve soil organic carbon and available nutrients，alleviate nitrogen deficiency， and enhance soil enzyme activities related to carbon and nitrogen cycling in degraded grasslands in this region. However， it also strengthened the carbon limitation of surface soil microorganisms.

Key words：Legume;Degraded grasslands;Available nutrients;Extracellular enzyme activities

草地在维持生物多样性、降低水土流失和保持土壤肥力方面发挥关键作用1，中国约 90% 天然草地出现退化迹象，其中超过 60% 达到中到重度退化程度[2]。（剩余22778字）