外源脱落酸对祁连山野生药食植物黄参代谢物的影响

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中图分类号：S567.53 文献标识码：A 文章编号：1007-0435（2026）01-0118-14

Abstract：This study aims to explore the effects of exogenous ABA on the accumulation pattrn of differential metabolites in the roots of wild medicinal-edible plants Sphallrocarpus gracilis of Qilian Mountains.Ultrahigh liquid phase system and ultra-high performance liquid chromatography-electrospray-triple quadrupole linear ion trap-tandem mass spectrometry （UPLC-ESI-QTRAP-MS/MS） extensive targeted metabolomics technology was used to detect and analyze the changes of metabolites in roots by applying with 20μg⋅L-1 exog enous abscisic acid（ABA）aswell as 2000mg⋅L-1 sodium tungstate.The results showed that two kinds of carotenoids were detected in the roots of Sphallerocarpus gracilis for the first time，they were β -carotene and lutein，respectively.Furthermore，atotalof 537 differential DAMs in7categories were identified，among them shikimic acid and phenylpropionic acid（206 species），terpenoids（93 species），alkaloids（92 species）and amino acids （84 species）accounted for a relatively high proportion.Pathway enrichment analysis was performed on differential metabolites based on Kyoto Encyclopedia of Genesand Genomes.The diferential metabolites （DAMs）were distributed in 15 metabolic pathways in 5categories，which were mainly enriched in phenylalanine metabolism，thiamine metabolism and tyrosine metabolism.In summary，exogenous ABA and its inhibitor sodium tungstate can affct the metabolic pattern of Sphallerocarpus gracilis，which is expected to be applied to the development of targeted regulators on key metabolic pathways.

Key words：Sphalerocarpus gracilis;Metabonomics;Abscisic acid; Diferential metabolites; Pathway enrich ment analysis

黄参（Sphallerocarpusgracilis）为伞形科迷果芹属的单种植物，在我国西北、东北、华北地区有少量分布，在甘肃境内，集中分布在海拔 2400～2900m 的张掖市山丹县祁连山支脉焉支山。（剩余25505字）