偏关首蓿MsMYB44基因的克隆、亚细胞定位 及表达分析

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中图分类号：Q943 文献标识码：A 文章编号：1007-0435（2026）01-0033-10

Abstract： The MYB（V-myb Avian myeloblastosis viral Oncogene Homolog） gene family represents one of the most extensive groups of transcription factors in plants，playing a crucial role in various processes including plant growth，development，and stress responses. This study utilized Medicago satiua‘Pianguan’to investigate the characteristics of the MsMYB44 gene through methodologies such as gene cloning，bioinformatics analysis，subcellular localization，and expression pattern analysis. The findings revealed that the MsMYB44 gene of Medicago satiua‘Pianguan’ comprised 966 base pairs and encoded a total of 321 amino acids. The MsMYB44 protein exhibited a high degree of homology to the MtMYB44 protein in Medicago truncatula and was localized within the nucleus. The expression of the MsMYB44 gene was observed in various tissues of Medicago satiua‘Pianguan’ across diferent developmental stages，indicating tissue-specific expression patterns.The MsMYB44 gene Was responsive to drought，salt stress，and abscisic acid（ABA） treatment.Under drought conditions，the relative expresson level of MsMYB44 initially increased，subsequently decreased，and then increased again，peaking at 12 hours. In response to salt stress，the relative expression level of MsMYB44 gradually increased after 48 hours，reaching its maximum at 72 hours.Following ABA treatment，the relative expression level of MsMYB44 peaked at 12 hours and then progressively declined. These findings would provide a theoretical framework for elucidating the role of the MsMYB44 gene of Medicago satiua in mediating the abiotic stress response.

Key Words：MsMYB44;Medicago sativa‘ Pianguan’ ; Cloning identification; Subcelllar localization; Expres sion pattern;Abiotic stress

全球气候不断变化，自然灾害频发，加剧了非生物胁迫对植物的不利影响[1]。（剩余17767字）