苎麻谷胱甘肽S-转移酶家族全基因组鉴定及其对镉胁迫的响应

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中图分类号：S813.3 文献标识码：A 文章编号：1007-0435（2026）01-0010-13

Abstract：Glutathione S-transferases （GSTs）playan important role in the regulation of plants against stressful environments，but the systematic studies on Ramie were lack.In this paper，a totalof43 BnGSTs gene families were identified by bioinformatic analyses，most of which were predicted to be located in the cytoplasm and belonged to the plant-specific Tau and Phi class Inter-species covariance analysis revealed that BnGSTs genes represented more orthologous genes in dicots than monocots；intra-species covariance analysis revealed that there were five pairs of paralogous genes among BnGSTs ，all of which were derived from the whole genome or fragmentary gene duplication，and allof them were found that Ka/Ks ratios were less than 1，indicating that they were subjected to purifying selection.Cis-acting element prediction revealed that the upstream of these

BnGSTs genes was rich in light-responsive，phytohormone-regulated and stre-responsive elements combined with transcriptome expression data， nine tissue-specific candidate genes were screened with significant （ P< 0.05）changes on expression patterns in roots，stems and leaves under cadmium stress. Two genes，BnGST22 and BnGST12，were identified as having significant up-regulation and high relative expresson，which could be used as target genes for further functional verification.This study further enriched the functional gene pool of ramie and provided a theoretical basis for the use of anti-stress resources and variety selection.

Key Words：Ramie; Glutathione S-transferases;Cadmium stress;Bioinformatic analysis

谷胱甘肽S-转移酶（GlutathioneS-Transfer-ases，GSTs）是催化多种外来物质与还原型谷胱甘肽（GSH偶联的超家族二聚体蛋白酶。（剩余20119字）