棘木内生真菌HP-12对结肠癌细胞增殖的抑制作用

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中图分类号：S794.908 文献标志码：A 文章编号：1004-390X（2025）05-0097-08

the inhibitory effects of fermentation broth on proliferation of colon cancer cels. [Methods] Moringa seeds were collected from Jinghong， Yuanjiang， and Yuanmou， Yunnan Province， and the endophytic fungi were isolated， purified， and cultured for fermentation. The MTT method was used to detect the effects of different endophytic fungi fermentation broth on the proliferation of human colon cancer HCT116 cels. [Results] A total of 122 endophytic fungi strains were obtained from moringa seeds. After the treatment of HCT116 cells with the dichloromethane extract from fermentation broth （DEFB） of endophytic fungi， 11 strains of endophytic fungi were screened out to significantly inhibit the proliferation of HCT116 cells.The DEFB from these obtained strains Was further used to treat NCM460 cels， human colorectal epithelial cells，and the HP-12 isolate showing weak inhibition of NCM460 cel proliferation was identified. Morphological and phylogenetic analyses preliminarily identified HP-12 as belonging to genus Lachnellula sp. HP-12 DEFB significantly inhibited the proliferation of HCT116 cells， with a half maximal inhibitory concentration （IC50） of 29.40μg/mL 5 whereas there was no significant effect on NCM46O cells proliferation. After treatment with HP-12 DEFB，HCT116 cells became wrinkled and round， and their ability to adhere to the substrate was dramatically decreased. Compared with the control group， in the treatment groups with HP-12 DEFB （30， 45， and 60μg/mL ）， the activity of Na/K -ATPase was significantly decreased，and the free fatty acid content was significantly increased.HP-12 DEFB had good thermal stability at 90∘C and below， and long-term ultraviolet radiation （UV） did not afect its inhibitory activity against colon cancer cells.

[Conclusion] A strain of Lachnellula sp.， HP-12， was obtained from moringa seeds. The fermentation broth of HP-12 significantly inhibits HCT116 colon cancer cell proliferation，and it is also stable underhigh temperature and long-term UV radiation conditions.

Keywords： moringa seeds; endophytic fungi; colon cancer; inhibition of proliferation; free fatty acids

结肠癌是第3大常见癌症，也是癌症相关死亡的第2大病因[1。（剩余12263字）