饥饿复投喂对昆明裂腹鱼生长和代谢的影响

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中图分类号：S964.9 文献标志码：A 文章编号：1004-390X（2025）05-0066-10

Abstract： [Purpose] To investigate the effects of different re-feeding models following starvation on the growth and metabolism of Schizothorax grahami. [Methods] A total of 144S. grahami with an average body weight of （51.96±0.46） g were selected for this study. Four feeding models were set： periodic fasting for one day， two days，three days followed by feeding for six days （model 1）， five days （model 2）， four days （model 3）， respectively， and continuous feeding were set as control group. Samples were collected on days seven and 14 for measurement of serum biochemical parameters and expression of metabolism-related genes， and the observation of histological characteristics of liver and intestines Was carried out. [Results] 1） Model 1 treated for seven days induced compensatory growth in S. grahami; model 2 and model 3 resulted in negative growth， with a significant reduction in hepatobody ratio after 14 days. 2） Feeding under model 3 resulted in significantly higher triglyceride （seven days） and total cholesterol （14 days） levels in S. grahami compared to the control group. 3） The re-feeding treatment after starvation at seven and 14 days significantly affected intestinal histological structure. Villus height， villus width and muscularis thickness of intestine were different from the control group. 4） After seven days of starvation re-feeding， g6pcla， pparb， lpl， and pparaa expression levels of liver in S. grahami under model 3 were significantly higher than other groups; after 14 days of starvation re-feeding， g6pcla and lpl expression levels under model 3 remained significantly higher than other groups，but pparaa expression level in the control group was significant higher than other groups. [Conclusion] All three starvation re-feeding models affect the growth and metabolism of S grahami to varying degrees. Among these， the model involving starvation for three days followed by re-feeding for four days significantly impacts the normal physiological functions of S⋅ grahami. Consequently， starvation for three days is the critical threshold for metabolic regulation in S. grahami.

Keywords： Schizothorax grahami； starvation re-feeding; histological characteristics; physiological status; gene expression

昆明裂腹鱼（Schizothoraxgrahami）隶属于鲤科（Cyprinidae）裂腹鱼亚科（Schizothoracinae）裂腹鱼属（Schizothorax），是中国特有的高原冷水性底栖鱼类，其适宜生长水温为 12～20°C ，主要集中分布于西南地区的高原峡谷河流和湖泊中[1，具有显著的季节性栖息特征。（剩余14438字）