羌活叶斑病病原鉴定及其生物学特性

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中图分类号：S435.672 文献标志码：A 文章编号：1004-390X（2025）05-0053-07

Abstract： [Purpose] To clarify the pathogen and biological characteristics of Hansenia weberbaueriana leaf spot disease，providing theoretical basis for prevention and control of the disease. [Methods]Diseased leaves of H. weberbaueriana planting base in Yulong Naxi Autonomous County，Lijiang City， Yunnan Province were used as research materials， the pathogen was isolated， purified， and identified by morphology; and ITS sequence phylogenetic analysis， pathogen determination and biological characteristics research were carried out. [Results]Morphological identification showed that the colony of isolated pathogen was round， covered with white mycelium on the front， dark brown on the back， with concentric rings; mature conidia were rod-shaped， with 2-3 septa， and the spore size was （10.52-20.54） μm×（3.24-4.32） μm .Based on ITS sequence alignment and phylogenetic analysis， the strain was identified as Phoma insulana. The pathogenicity test of pathogen confirmed the pathogen could cause H. weberbaueriana leaf spot disease. The results of biological characteristics showed that PDA medium， pH7.0 ， 25°C and all dark culture conditions were conducive to strain growth and conidia formation. The sporulation quantity was the highest when glucose was used as carbon source， and the mycelial growth was the fastest when sucrose was used as carbon source. When peptone was used as nitrogen source， sporulation and mycelial growth were the most significant. [Conclusion] P .insulana is the pathogen causing H. weberbaueriana leaf spot. The culture conditions of pH 7.0， 25°C and darkness are suitable for pathogen growth and conidia formation.

Keywords： Hansenia weberbaueriana; leaf spot disease;Phoma insulana; biological characteristics

羌活（Hanseniaweberbaueriana）为伞形科（Ap-iaceae）羌活属（Hansenia）多年生草本植物，具有抗炎、抗血栓、镇痛等药用功效1]，可内服外用治疗风热感冒、湿肿等多种疾病[2]。（剩余10520字）