高抗性淀粉水稻品种功米3号的淀粉—脂质调控网络

打开文本图片集

中图分类号：S511.032 文献标志码：A 文章编号：1004-390X（2025）05-0014-12

Abstract： [Purpose] To reveal the key genes and regulatory pathways controlling the formation and accumulation of resistant starch （RS） in the grains of high-RS rice variety Gongmi 3，providing a theoretical basis for the breeding high-quality functional rice varieties with high RS content.

[Methods] The high-RS content rice variety Gongmi 3 and the low-RS content rice variety Diantun 50 were used as objects and control， respectively. Transcriptome sequencing and comparative analysis were carried out at multiple periods （the1Oth，17th and 24th day after flowering） to reveal the molecular regulatory mechanism of RS formation in Gongmi 3. [Results] In the two varieties， the number of differentially expressed genes （DEGs） on the 24th day after flowering was substantially higher than that of the previous two stages. GO and KEGG enrichment analysis showed that lipid transport and binding terms were significantly enriched across alltree stages， the corresponding DEGs predominantly encoded non-specific lipid transport proteins， regulating lipid accumulation. Starch and sucrose metabolism pathway were significantly enriched at the 1Oth and 24th day after flowering， with glycosyl hydrolase genes as the main DEGs， regulating the content of reducing sugars. Venn diagram analysis identified 635 genes consistently differentially expressed in all three developmental stages，annotation and enrichment analysis of these genes showed significant enrichment for the lipid transport and binding items. Among these， six key genes， including a novel gene （Oryza_ sativa_newGene_1652）， were consistently upregulated in Gongmi 3，serving as critical candidate genes for regulating RS content in subsequent functional validation. [Conclusion] It is speculated that RS accumulation in Gongmi 3 grains is coordinately regulated by lipid metabolism and starch biosynthesis pathways， likely through enhanced formation of RS5 （starch-lipid complexes），and the 3rd developmental stage serves as a critical window orchestrating RS accumulation.

Keywords： Gongmi 3; resistant starch; transcriptomics; lipid metabolism

淀粉是人类膳食中碳水化合物的主要来源，是世界上最重要的多用途多糖类物质[1]。（剩余17497字）