水稻OsS1FA对热胁迫的响应机制

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Abstract：S1FA proteins are a class of transcription factors that play significant roles in plant growth and development as wellas in tolerance toabiotic stressIn this study，two S1FA family genes were identified inrice （Oryza sativa L.），and their biological functions in the response to heat stress were investigated.Bioinformatics analysis indicated that OsS1FA40 （LOC_Os04g33440） and OsS1FA20 （LOC_Os04g33420）genes have simple structures and high sequence homology. Expression pattern analysis revealed that OsSIFA40 is expressed in all tissues examined，whereas OsS1FA20 is predominantly expressed in the endosperm.Double mutant material of OsSIFA40 and OsS1FA20 in japonica rice Nipponbare （NIP）were obtained by using CRISPR-Cas9. Thermotolerance of Osslfas mutants seedlings under heat treatment （ 42∘C ， RH>90% ， 48h ），theOsslfas mutants exhibited a more thermotolerant phenotype compared to the wild type plants，with relatively less disruption to leaf cell morphology and structure.After recovery at 28∘C for one week，the mortality rate of the Osslfas mutants was lower than that of the WT plants.The analysis of physiological and biochemical related indicators showed that after heat treatment，the contents of chlorophyllA，chlorophyll B，total chlorophyll， soluble sugar and maximum photosynthetic efciency in the Oss1fas mutants were higher than those in the WT plants，indicating that heat stressactivated the antioxidant mechanism and osmotic stress response of the Oss1fas mutants，and also affected the photosynthetic system.Furthermore， phenotypic analysis during plant development showed that the Oss 1fas mutants displayed narrow and short leaves，as wellas reduced root length， indicating potential involvement of these genes in leaf morphogenesis and plant growth regulation. In conclusion， OSIFA in rice negatively regulate thermotolerance. This study provide a theoretical basis for deciphering the function of OsS1FA in regulating the response to high-temperature stressand for molecular breeding.This research holds significant implications and genetic improvement value for enhancing plant thermotolerance.

Key words： OsS1FA20; OsSIFA40; heat stress; expression analysis; functional identification; transcriptome sequencing

植物的生长发育受到多种生物胁迫和非生物胁迫的影响。（剩余20803字）