甘蓝型油菜苗期高光效种质资源的筛选与鉴定
打开文本图片集
Abstract：Brassica napus L.is one of the most important oilseed crops in China and plays a vital role in securing the national supply of edible vegetable oil.Enhancing photosynthetic efficiency represents a key strategy forachieving high yieldand sustainable，resource-efficient breeding in rapeseed.In this study，We used 245 Brassica napus germplasm resources as materials，measuring 19 indicators including photosynthetic parameters， photosynthetic pigment，biomass，and chlorophyll fluorescence parameters at the seedling stage.Significant variations were observed among germplasms in gas exchange，PSII （photosystem II） energy conversion，and thermal dissipation，demonstrating rich geneticdiversity and substantial potential for selection.Stomatal conductance and transpiration rate showed highly significant positive correlations with fresh weight and the chlorophyllfluorescence parameters Pi_Abs （performance index on an absorption basis） and Ss（complementary area above the O-Jphase of the fluorescence induction curve），but were extremely negatively corelated with ETo/RC（electron transport flux per reaction center）. These relationships suggest that coordinated regulation betweenphotosynthetic activity and water use plays a critical role in biomassaccumulation.Moreover，biomass wassignificantly and positively correlated with Pi_Absand Ss，indicating that chlorophyll fluorescence parameters can serve as reliable indicators of photosynthetic system eficiency and growth potential.A comprehensive evaluation model （D-value）was developed through principal component analysis combined with membership function analysis，enabling the establishment of an integrated assessment framework for high light-use efficiency in B .napus. The 245 germplasm accessions were classified into five groups by clusteranalysis35 high-efficiency，52 medium-high-eficiency，49 intermediate，88 medium-lowefficiency，and 21 low-efficiency types，and established a high-light-efficiency evaluation model.Twelve high light-efficiency germplasm accessions were ultimately identified.The evaluation system established in this study provides an effective approach for preliminary screening of high-efficiency germplasm resources and offers a theoretical foundation and data support for the discovery and breeding of rapeseed varieties with enhanced light-use efficiency.
Key words： Brassica napus L.; seedling stage;high photosynthetic eficiency ; photosynthetic performance chlorophyll fluorescence parameters
油菜（BrassicanapusL.）是世界三大油料作物之一，是我国最主要的油料作物，常年种植面积超过6667万 hm2 （1亿亩），为我国贡献了约 50% 的植物油产量[1]。（剩余16402字）
