利用全基因组关联分析鉴定小麦籽粒钙含量相关的遗传位点和候选基因

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Abstract：Calcium is an essential mineral for human health.As a staple food crop worldwide，the calcium content in wheat grains directly influences human nutrition.Biofortification represents a highly cost-effective strategy to enhance calcium content in wheat grains and address dietary calcium deficiency.However，the molecular mechanisms regulating calcium content in wheat grains remains poorly understood. In this study，we measured grain calcium concentration of 349 wheat varieties （lines） and conducted a genome-wide association study（GWAS）using the Wheat 660K SNP array.A total of 523 significant SNPs associated with grain calcium content were identified，with most distributed on chromosomes 4A and 6B.Among these，76 SNPs were consistently detected in four or more replicates.Haplotype analysis showed thatGCaC_Hap_4Aand GCaC_Hap_6B represent potential key loci regulating grain calcium content. Further bioinformatics analysis suggested TraesCS4A03G0985800 as a promising candidate gene for the GCaC_Hap_4A locus.This findings provide valuable insights into the genetic basis of grain calcium content in wheat and ofer useful references for molecular breeding.

Key words ： wheat; grain calcium content; genome-wide association study;candidate gene analysis

矿物质元素作为人体必需的营养物质，在维持机体正常生理功能、促进代谢以及预防疾病等方面发挥着极其重要的作用[1。（剩余16908字）